ACTA AGRICULTURAE BOREALI-SINICA ›› 2014, Vol. 29 ›› Issue (6): 84-93. doi: 10.7668/hbnxb.2014.06.016

Special Issue: Corn

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Analysis of Genetic Diversity of 82 Maize Inbred Lines

SHI Hai-chun, YUAN Hao, LI Dong-bo, YU Xue-jie, KE Yong-pei   

  1. College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China
  • Received:2014-07-08 Published:2014-12-28

Abstract: The evaluation of germplasm resources diversities and the genetic relationships among germplasm have been an important part of maize breeding research.In this study,genetic diversities of 82 inbred lines were analyzed by morphological and SSR markers.The results showed that the average Euclidean distance calculated with 15 phenotypic characters was 50.57;the variation in the range was 9.05-146.23,indicating that the genetic diversity of these inbred lines was rich.All the inbred lines could be divided into six groups with the genetic distance of 38.06 as an indicator.The clustering was not highly consistent with the pedigrees.Based on stable amplification profiles obtained and significant polymorphic,63 pairs of SSR primers were selected to detect 601 alleles,and the average number of alleles per locus was 9.5 with a range from 4 to 24,and the values of polymorphism information content (PIC) for each SSR locus varied from 0.454 6 to 0.916 9 with an average of 0.752 9.The genetic similarities of SSR marker pattern ranged from 0.544 1 to 0.933 4 with an average of 0.667 3.The 82 inbred lines were divided into seven groups according to their genetic similarity by bounded coefficient of 0.673 5,among which inbred lines belonged to five family groups occupying 84.1%.Among these inbred lines,32.9% were Reid group,23.2% PB,13.4% Lancaster,7.3% Tangsipingtou,and 7.3% Lüdahonggu group,with the other inbred lines divided into two groups occupying 11.0% and 4.9%,respectively.The clustering was consistent with its genealogical sources.

Key words: Maize, Inbred line, Morphological markers, SSR markers, Genetic diversity

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Cite this article

SHI Hai-chun, YUAN Hao, LI Dong-bo, YU Xue-jie, KE Yong-pei. Analysis of Genetic Diversity of 82 Maize Inbred Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 84-93. doi: 10.7668/hbnxb.2014.06.016.

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