Correlation Analysis Polymorphism of GnRHR Gene and Litter Size of Laoshan Dairy Goats

doi:10.7668/hbnxb.2014.06.015

Abstract

Abstract: The objectives of the present study were to elucidate the polymorphism of GnRHR gene and its relationship with litter size of Laoshan dairy goat.Seven pairs of primers were designed to detect single nucleotide polymorphism (SNP) of GnRHR gene in Laoshan dairy goat with different kids by PCR-RFLP and PCR-SSCP.The results showed two mutations (A261G and G55A) were detected.For A261G locus in exon1,the does with genotype GA had 0.06 and 0.04 kids more than those with genotype AA and GG in primipara ewes,but the differences were not significant (P>0.05);the does with genotype GA had 0.35 and 0.45 kids more than those with genotype AA and GG in multiparity ewes (P>0.05).For G55A locus,two genotypes (GG and GA) were detected,the does with genotype GG had 0.13 and 0.19 more than those with genotype GA in primipara ewes and multiparity ewes,respectively.However the differences were not significant (P>0.05).These results preliminarily indicated that A261G locus of GnRHR gene is a potential molecular marker for improving litter size in dairy goats.

Key words: GnRHR gene, Dairy goats, Litter size

CLC Number:

S826.03

LIU Nan, MA Xiao-li, CHENG Ming, LIU Kai-dong, HE Jian-ning, LIU Ji-feng. Correlation Analysis Polymorphism of GnRHR Gene and Litter Size of Laoshan Dairy Goats[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 78-83. doi: 10.7668/hbnxb.2014.06.015.

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