Abstract: In order to screen the best modeling method of Lung-Qi deficiency mice and to detect its changes of immune function,the model of Lung-Qi deficiency mice was established by SO₂ method,SO₂ +chill factor stimulation,cigarette-smoking method,and cigarette smoking+chill factor stimulation,respectively.And the indexes such as body weight,lung necropsy and histopathology were detected to screen the best modeling methods.The immunity indexes such as the activity of peritoneal macrophage,the ability of B-cell antibody productionin vitro,the quantity of IL-2,IL-4,IL-6,IL-10,IFN-γ,TNF-α and NO in serum,the sIgA and sIgM levels in lung homogenate and the lymphocyte proliferation were also detected.The results showed that the mice after being modeled by smoking cigarettes+chill factor stimulation method had the more serious pathological changes.The changes of lung hemorrhage,lung necrosis and inflammatory cell infiltration in the group using smoking cigarettes+chill factor stimulation method were severer than the other groups.Compared to the control group,the body weight,spleen index and thymus index of the mice using smoking cigarettes+chill factor stimulation method decreased significantly by 18.35%,37.12% and 19.69% respectively,and the lung index significantly increased by 12.73%.The activity of peritoneal macrophages(P<0.01)and the quantity of NO(P<0.05)in Lung-Qi deficiency model mice decreased significantly,while the proliferation activity of T and B lymphocytes increased significantly.The quantity of IL-2(P<0.01),IFN-γ and IL-4(P<0.05)increased,and IL-6 decreased(P<0.05).The quantity of sIgA and sIgM increased significantly.The results suggested that cigarette smoking+chill factor stimulation was superior to other three methods,and the immunological function was significantly damaged.

Key words: Lung-Qi deficiency, Cigarettes smoked, SO₂, Mice model, Immune function

CLC Number: 

• S829