Effect of ORexins( HypocRetin) A on the ExpRession of OX1R in Ovion Iuteinized GRanulosa Cells

doi:10.7668/hbnxb.2013.06.017

Abstract

Abstract: To observe the effect of orexin A on the expression of OX 1 R in ovine luteinized granulosa cells in vitro,the cells were treated with different concentrations (0.05,0.2,0.5,1 μg/mL) of orexins A.Then the total RNA was extracted from the cells after treated in 0,2,6,12 h,and the mRNA expression levels of OX1R were eval-uated using reverse transcription quantitative PCR.In result,orexin A induced release of OX1R in ovine luteinized granulosa cells.In high concentration (1 μg/mL) group,the OX1R mRNA expression level was increased to the highest in 2 h(P<0.01) and gradually weakened since then;in low concentration(0.05,0.2,0.5μg/mL) group, the OX1R mRNA expression level was increased in 12 h and the expression level of 0.2 μg/mL group was in-creased to the highest in 12 h(P<0.01).Orexin A induced expressions of OX1R mRNA,the group of 1 μg/mL was more rapid up regulation compared with the low concentration (0.05,0.2,0.5 μg/mL) group.

Key words: ORexin A, OX1R, Sheep, Luteinized gRanulosa cells

CLC Number:

S826

LI Wen-jiao, DU Chen-guang, JIAN Rui-zhen, WEN Shi-yong, WANG Wei-ping, SUN Bo, XU Xiao-jing, LIU Yong-zhi. Effect of ORexins( HypocRetin) A on the ExpRession of OX1R in Ovion Iuteinized GRanulosa Cells[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 98-103. doi: 10.7668/hbnxb.2013.06.017.

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