Identification of Cis-element Location in AtHAK5 PRomoteR

doi:10.7668/hbnxb.2013.06.016

Abstract

Abstract: AtHAK5 encodes a high affinity K +transporter.Six AtHAK5 promoter fragments with different length were cloned into the upstream of β-Glucuronidase reporter gene ( GUS) in pORE R1 binary expression vector, which forms promoter AtHAK5∶∶GUS fusion gene.The T3 generation transformed seeds stably expressing the fusion gene were gotten through Agrobacterium-mediated Arabidopsis transformation with floral dip method.The cis-elements of the AtHAK5 promoter was located at the transcription region of 471-267 thought GUS activity based histochemical staining of the transformed seedlings grown on low potassium condition.This study identified the functional domain within the AtHAK5 promoter,which sets the foundation for further finding of the corresponding transcript factor.

Key words: ARabidopsis, AtHAK5 gene, PRomoteR, Cis-element

CLC Number:

Q943
S184

WANG Xin, YANG Ju, QI Zhi, ZHANG Yan-tao. Identification of Cis-element Location in AtHAK5 PRomoteR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 93-97. doi: 10.7668/hbnxb.2013.06.016.

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http://www.hbnxb.net/EN/Y2013/V28/I6/93

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