Abstract: Rice blast disease is the severest disease in rice production. The formation of appressorium plays a key role on disease extending when pathogenic fungi intrudes into rice. The formation of appressorium needs a P-type ATPase. Small molecules from the host are able to enter into the pathogen during the interactions between pathogen and host,to reach the purpose of anti-pathogen infection. In this study,a 232 bp DNA sequence from the first exon of the P-ATPase gene,MgAPT2,was used as interfering fragment and inserted into interfering vector posi-tively and negatively. The interfering vector was then transformed into rice variety Nipponbare susceptible to rice blast.Rice blast resistance of transgenic plants and gene expression were checked. The results showed that the ex-pression level of the P-ATPase gene MgAPT2 from Magnaporthe grisea in transgenic rice decreased and blast-dis-ease resistance of transgenic rice increased. This study provided a new way for innovation of rice blast-disease resist-ance germplasm.

Key words: Rice, Blast resistance, Gene silencing

CLC Number: 

• S511