Cloning and Analysis of Two New PR10 Members in Soybean

doi:10.3969/j.issn.1000-7091.2013.04.002

Abstract

Abstract: Two new soybean genes named Glyma09g04530 and Glyma15g15590 which encoding PR10 proteins were cloned from a SMV resistant cultivar Kefeng No． 1． Glyma09g04530 and Glyma15g15590 have a 477 bp ORF and coding 158 amino acids． Sequence alignment and evolutionary tree clustering analysis showed that Gly-ma09g04530 and Glyma15g15590 are new PR10 protein genes in soybean． The genes of Glyma09g04530 and Gly-ma15g15590 expressed in root， stem and leave by using Real- time PCR analysis in soybean． Expression level of Gly-ma09g04530 and Glyma15g15590 were strongly induced and highly expressed in leaves of Kefeng No． 1 after inocu-lation SMV． We speculate that Glyma09g04530 and Glyma15g15590 may be involved for getting resistance to SMV．Key words: Soybean; PR10 protien; Gene cloning; Expression analysis

Key words: Soybean, PR10 protien, Gene cloning, Expression analysis

CLC Number:

CHEN Hua-tao, CHEN Xin, GU He-ping, ZHANG Hong-mei, YUAN Xing-xing, CUI Xiao-yan. Cloning and Analysis of Two New PR10 Members in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 7-12. doi: 10.3969/j.issn.1000-7091.2013.04.002.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2013.04.002

http://www.hbnxb.net/EN/Y2013/V28/I4/7

References

[1] Gianinazzi S，Martin C，Valée J C，et al．Apparition de nou-velles macromol cules lorsde la répression de la synthèse vi-rale[J].CR Acad Sci Paris，1970，270D: 2383－2386．
[2] Van Loon L C．Pathogenesis-related proteins[J].Plant Mol Biol，1985,4: 111－116．
[3] Antoniw J F，Ritter C E，Pierpoint W S，et al．Comparison of three pathogenesis-related proteins from plants of two culti-vars of tobacco infected with TMV[J].J Gen Virol，1980，47:79－87．
[4] Shan D M．Genetic engineering for fungal and bacterial dis-eases[J].Current Opinion in Biotechnology，1997，8 (2):208－214．
[5] Uritani I．Protein changes in diseased plants[J].Ann Rev Phytopathology，1971，9: 211－234．
[6] Guevara-morato M A，De lacoba M G，Garc a-luque I，etal．Characterization of a pathogenesis-related protein 4(PR-4) induced in Capsicum chinense L3 plants with dual RNase and DNase activities[J].J Exp Bot，2010，61(12): 3259－3271．
[7] Rogers E E，Ausubel F M．Arabidopsis enhanced disease susceptibility mutants exhibit enhanced susceptibility to several bacterial pathogens and alterations in PR-1 gene expression[J].Plant Cell，1997，9: 305－316．
[8] Christensen A B，Chol B H，Nasby M，et al．The molecular characterization of two barley proteins establishes the no-vel PR-17 family of pathogenesis-related proteins[J].Mo-lecular Plant Pathology，2002，3(3): 135－144．
[9] Gennady P，Moiseyeva，Larisa I，et al．Primary structures of two ribonucleases from ginseng calluses: New members of the PR-10 family of intracellular pathogenesis-related plant proteins[J].FEBS Letters，1997，407 (2): 207－210．
[10] Pulla R K，Lee O R，Ina J G，et al．Expression and func-tional characterization of pathogenesis-related protein family 10 gene，PgPR10-2，from Panax ginseng C．A．Meyer[J].Physiological and Molecular Plant Pathology，2010，74: 323－329．
[11] Clive S C，Hipskind J D，Nicholson R L．cDNA cloningof a Sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes followinginoculation with cochliobolus heterostrophus or colletotri-chum sublineolum[J].Molecular Plant-Microbe Interac-tions，1999，12(6): 479－489．
[12] Katil S O，Perumal R，William L，et al．Expression of pathogenesis-related protein PR-10 in sorghum floral tis-sues in response to inoculation with Fusarium thapsinum and Curvularia lunata[J].Molecular Plant Pathology，2010，11(1): 93－103．
[13] 郭迟鸣，毛倩，杨晓坡，等．水稻 XIOsPRI O 基因表达调控的初步研究[J]. 西北植物学报，2010，30(9):1721－1727．
[14] 陈新，易金鑫，张红梅，等．小豆抗病相关基因 Va-PR3 的克隆与表达分析[J]. 江苏农业学报，2009，5:1119－1123．
[15] Chen H T，Chen X，Gu H P，et al．Disease-resistant transgenic adzuki bean plants obtained through an effi-cient transformation system[J].Crop and Pasture Sci-ence，2013，http://dx．doi．org/10．1071/CP12300．
[16] Michel L，Kauffmann S，Geoffroy P，et al．Biological function of pathogenesis-related proteins: Four tobacco pathogenesis-related proteins are chitinases[J].PNAS，1987，84(19): 6750－6754．
[17] 杜良成，王钧．病原相关蛋白及其在植物抗病中的作用[J]. 植物生理学通讯，1990(4): 1－6．
[18] Ross A F．Systemic acquired resistance induced by local-ized virus infections in plants[J].Virology，1961，14:340－358．
[19] Van Loon L C，Van Strien E A．The families of pathogen-esis related proteins，their activities，and comparative a-nalysis of PR-l type proteins[J],Physiological and Mol-etular Plant Pathology，1999，55: 85－97.
[20] Aglika E．Pathogenesis-related proteins: research pro-gress in the last 15 years[J].Gen Appl Plant Physiolo-gy，2005，31(1－2): 105－124．
[21] Park C J，Kim K J，Shin R，et al．Pathogenesis-relatedprotein 10 isolated from hot pepper functions as a ribo-nuclease in an antiviral pathway[J].Plant J，2004，37(2): 186－98．
[22] Kleine-tebbe J，Wangorsch A，Vogel L，et al．Severe oralallergy syndrome and anaphylactic reactions caused by aBet v 1-related PR-10 protein in soybean，SAM22[J].The Journal of Allergy and Clinical Immunology，2002，110(5): 797－804．
[23] Graham M Y，Weidner J，Wheeler K，et al．Induced ex-pression of pathogenesis-related protein genes in soybeanby wounding and the Phytophthora sojae cell wall glucan elicitor[J].Physiological and Molecular Plant Patholo-gy，2003，63(3): 141－149．
[24] 陈华涛，陈新，顾和平，等．大豆 GmPR10 基因克隆与植物表达载体的构建[J]. 江苏农业学报，2011，27(3): 494－499．
[25] Sirkorski M M，Szlagowska A E，Legocki A B．Structure of Lupinus luteus genes YPR-10．1a and YPR-10．1b en-coding two homologous pathogenesis-related proteins ofPR-10 class[J].Plant Physiol，1998，116: 192－195．
[26] Sikorski M M，Biesiadka J，Kasperska A E，et al．Expres-sion of genes encoding PR10 class pathogenesis-related proteins is inhibited in yellow lupine root nodules[J].Plant Sci，1999，149: 125－137．
[27] Zhou X J，Shan L，Xu Y H，et al．A cotton cDNA(GaPR-10) encoding a pathogenesis-related 10 protein with in vitro ribonuclease activity[J].Plant Sci，2002，162(4):629－636．
[28] Liu X Y，Huang B B，Lin J，et al．A novel pathogenesis-related protein (SsPR10 ) from Solanum surattense withribonucleolytic and antimicrobial activity is stress-and pathogen inducible[J].Journal of Plant Physiology，2006，163(5): 546－556

Please choose a citation manager

Content to export