Cloning and Expression Analysis of Omasum Lysozyme Gene(TGOLyz) in Tibetan Goat

doi:10.3969/j.issn.1000-7091.2013.03.007

Abstract

Abstract: Based on species homology，the lysozyme cDNA was cloned from omasum in Tibetan goat by PCR and RT-PCR，and named TGOLyz gene． The sequence of nucleotides and deduced amino acids of TGOLyz was analyzed through bioinformatics methods，and the gene expression levels at five tissues of Tibetan goat were detected through qRT-PCR． The result showed that the coding region of the TGOLyz cDNA was 444 bp，and encoded 147 amino acids with a molecular weight of 16.29 kDa and isoelectric point of 6.08． The deduced amino acids sequence of TGOLyz shared 95.24% homologies with that from cow stomach 1 and had 67.61% homologies with that from yak stomach． The phylogenetic analysis showed that the TGOLyz gene belongs to c-type lysozyme． Real-time quantitative PCR assay demonstrated that the TGOLyz could be detected in all 5 tissues with the highest expression level observed in omasum． All these would establish a foundation for TGOLyz of its molecular mechanism of antibacterial and digestive function．

Key words: Tibetan goat, TGOLyz gene, Clone, qRT-PCR, Bioinformatics

CLC Number:

Zhang Peng, Jiang Mingfeng, Ma Xiaorui, Fang Yi, Wang Yong. Cloning and Expression Analysis of Omasum Lysozyme Gene(TGOLyz) in Tibetan Goat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 35-42. doi: 10.3969/j.issn.1000-7091.2013.03.007.

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