Expression Analysis of IFSI Gene in Isoflavonoids Pathway in Soybean

doi:10.3969/j.issn.1000-7091.2013.03.005

Abstract

Abstract: Previous studies have shown that isoflavone synthase (IFS) encode key enzymes in the isoflavonoids pathway in Soybean．There are two IFS homologues IFS1 and IFS2 in Soybean，The expression level of IFS1 gene in different tissues will be quantified by fluorescence quantitative PCR． The results revealed that the transcripts were widely distributed in all the tested tissues．IFS1 gene showed the higher expression level in the tender organs: root，stem， leaf germinating 7 days than that in the older organs: roots，stems，leaves growing 70 days． Expression level in seeds at 25 days and 45 days post anthesis were also lower，while with higher content in the mature embryo，suggesting that IFS1 gene has finished the Soybean isoflavone accumulation process in the early embryo formation．Further，the analysis of expression induced by abiotic treatments indicated that the genes were differentially regulated under IAA，ABA，PEG，NaCl，cold and wounding treatments． It will provide more knowledges on isoflavone biosynthesis and resistance to abiotic stress in Soybean．

Key words: Soybean, Isoflavones, Isoflavone synthase, Gene expression

CLC Number:

Q786

Lian Yun, LI Haichao, Wang Shufeng, Wu Yongkang, Li Jinying, Lei Chenfang, Lu Weiguo. Expression Analysis of IFSI Gene in Isoflavonoids Pathway in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 25-29. doi: 10.3969/j.issn.1000-7091.2013.03.005.

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