Identification and Genetic Analysis of Downy Mildew Resistance in Brassica oleracea var． capitata L．

doi:10.3969/j.issn.1000-7091.2013.03.035

Abstract

Abstract: We confirmed that the pathogen of cabbage downy mildew was obligate cruciferae Hyaloperonospora parasitica，according to the symptom of the susceptive S101 in filed，and the result of pathogen microscopical examination and re-inoculating on susceptive materials having no disease． Then did the genetic analysis by natural induction in field and artificial inoculation in greenhouse， the proportions of resistant plants and susceptive plants were 0. 91 ∶ 1( in field) and 0．96∶ 1( in greenhouse) in the backcross population constructed by F1 crossing susceptive parent S101，as are in accordance with 1∶ 1 separation rate assayed by the Chi-square test． In the F2 separation population，the proportions were 2． 93∶ 1( in field) and 3.01 ∶1( in greenhouse) ，being in accordance with 3 ∶ 1 separation rate by the same test． Two kinds of resistance identification methods show that the resistance to downy mildew of these population is monogenic inheritance， so we could use the artificial inoculation in cross stage to identify the resistance to cabbage downy mildew in entire seedling stage simply and fastly．

Key words: Headed cabbage, Downy mildew, Resistance identification, Genetic analysis

CLC Number:

S635.03

Yu Li1, Huang Jianxin, Wang Hong, Wang Shenyun, Ding Wanxia, Li Jianbin. Identification and Genetic Analysis of Downy Mildew Resistance in Brassica oleracea var． capitata L．[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 193-198. doi: 10.3969/j.issn.1000-7091.2013.03.035.

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