Proteomics Analysis of Rice Root Senescence under Nutrient Stress Condition during Late Development Stage

doi:10.3969/j.issn.1000-7091.2013.02.003

Abstract

Abstract: In order to reveal the molecular senescence mechanism of rice root with nutrient deficiency during filling stage,the proteins expression profile of root in hybrid rice Weiyou 916 were investigated by using the ap-proach of proteomics. Proteins were extracted from rice roots and separated by two-dimensionalgel electrophoresis,then 23 differential expression proteins were obtained,among which 17 proteins involved respiration(2 proteins),root development(7 proteins),stress response(6 proteins) and hypothetical protein(2 proteins) were identified by ESI-Q MS/MS. The 17 proteins' functions were analyzed and results showed that:rice roots had fast development andgrowth at early nutrient deficiency. Long-term nutrient deficiency resulted in the accumulation of stress response proteins,weak respiration,slowgrowth and high lignification in rice root,which led to the senescence of root.

Key words: Rice(Oryza sativa), Nutrient deficiency, Root, Senescence, Proteomics

CLC Number:

S312
Q78

SHAO Cai-hong, LI Yao, QIAN Yin-fei, QIU Cai-fei, PENG Chun-rui, LIUguang-rong, XIE Jin-shui. Proteomics Analysis of Rice Root Senescence under Nutrient Stress Condition during Late Development Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 12-19. doi: 10.3969/j.issn.1000-7091.2013.02.003.

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