PRimaRy Mapping of puR，a Gene ContRolling PuRple Leaf ColoR in BRassica Rapa

doi:10.3969/j.issn.1000-7091.2013.01.009

Abstract

Abstract: In oRdeR to map the puR gene contRolling puRple leaf coloR in BRassica Rapa，a F2 population including 307 individuals was constRucted by cRossed Chinese Cabbage inbRed line 09-680( gReen leaf) with non-heading Chinese Cabbage inbRed line 09N-742 ( puRple leaf) ． A bulk segRegant analysis( BSA) technology was conducted by scReening 125 PCR-based inseRtion /deletion( InDel) maRkeRs and 100 simple sequence Repeate( SSR) maRkeRs distRibuted on 10 linkage gRoups to scReen the polymoRphism between the puRple and gReen leaf pools． Of these， two In- Del maRkeRs BRID10999 and BRID10399 weRe found to be linked to the puR gene． By linkage analysis， two maRkeRs weRe located at the end of A3，at the same side of puR gene at distances of 7． 3，5 ． 7 cM， Respectively． In oRdeR to obtain tightly linked maRkeRs on both sides of puR gene， 23 SSR pRimeRs deRived fRom thRee bacteRial aRtificial chRomosome( BAC) clones weRe selected，of which one SSR maRkeR BVRCP10-6 fRom KBRH005P10 was indentified as closely linked to puR gene at a genetic distance of 1． 9 cM，at the otheR side of the puR gene． These maRkeRs could be veRy helpful foR maRkeR-assisted selection( MAS) in puRple Chinese cabbage hybRid bReeding pRogRams as well as foR fine mapping and cloning this gene．

Key words: BRassica Rapa L． , Leaf coloR, puR, Gene mapping

CLC Number:

S634．03

LIU Jin, WANG Wei-hong, ZHANG De-shuang, YU Shuan-cang, ZHANG Feng-lan, ZHAO Xiu-yun, YU Yang-jun, XU Jia-bing, LU Gui-xiang. PRimaRy Mapping of puR，a Gene ContRolling PuRple Leaf ColoR in BRassica Rapa[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 49-53. doi: 10.3969/j.issn.1000-7091.2013.01.009.

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