Genetic DiveRsity Analysis of Soybean Elite CultivaR Basing on MoleculaR and Phenotype TRaits

doi:10.3969/j.issn.1000-7091.2013.01.004

Abstract

Abstract: Suinong 14 is the typical elite cultivaR including high-quality，high yield，disease Resistance and wide adaptability． Analysising the moleculaR and phenotypic genetic basis of Suinong 14 pedigRee，pRovide the theoRetical guidance in puRposely chosing hybRid paRent to bRoaden the genetic basis． In the cuRRent ReseaRch，50 agRonomic tRaits and 550 SSR maRkeRs weRe chosen and analyzed． AccoRding to natuRe of eveRy tRait， the 50 agRicultuRal tRaits weRe adivided into 6 gRoups，gRowth tRaits，yield tRaits，quality tRaits， Resistance tRaits． In total 1 494 alleles weRe detected in 550 SSR maRkeRs with an aveRage allele numbeR of 2． 716 4 peR locus，The aveRage of PIC is 0． 445 0，of which 30 high polymoRphic loci can be chosed as impoRtant locis to evaluate the genetic diveRsity of soybean geRmplasm ResouRces; LG C1 has the highest pRopoRtion of polymoRphic loci of 0． 961 5，LG A2 has the moRe conseRvative fRagment up to 11． At least two SSR loci weRe needed to discRiminate Suinong 14 fRom its pedigRee． In total 255 alleles weRe detected in 50 phenotypic with an aveRage allele numbeR of 5． 100 peR locus，The aveRage of PIC is 0. 683 2． PRincipal component analysis ( PCA) Result showed that the cumulative contRibute of 6 pRincipal components foR 50 agRonomic tRaits was above 80． 1%，Analysis of the composition of each pRiciple component show that eveRy tRaits have an impoRtant Role in the compRehensive study of genetic diveRsity of soybean geRmplasm． Some impoRtant tRaits of soybean weRe selected as key index in soybean study． The CoRRelation between SSR clusteR and agRonomic tRaits clusteR basing on UPGMA is 0． 393 0， the Result indicated that the two kinds of clusteRing methods can only Reveal some Relationship among the species at a ceRtain extent，Thus the optimal stRategy combined SSR data and agRonomic tRaits is necessaRy foR genetic diveRsity analysis of soybean geRmplasm．

Key words: Soybean, SSR, BackgRound, Genetic diveRsity

CLC Number:

S565．03

QIN Jun, ZHANG Meng-chen, CHEN Wei-yuan, CHANG Ru-zhen, QIU Li-juan. Genetic DiveRsity Analysis of Soybean Elite CultivaR Basing on MoleculaR and Phenotype TRaits[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 19-26. doi: 10.3969/j.issn.1000-7091.2013.01.004.

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