Cloning and Sequence Analysis of a DNA Encoding ACC Synthase Gene from Ficus carica

doi:10.3969/j.issn.1000-7091.2012.06.008

Abstract

Abstract: Specific primers were designed based on the the reported ACC synthase( ACS) gene sequences of Ficus carica in the GenBank． Using genome DNA extracted from Ficus caricafruits as template the specific PCR product of ACS was obtained， then the product was ligased to pGM-T easy vector with PCR， restriction enzyme digestion and sequencing． The sequencing data showed that the PCR product was 590 bp encoding 196 predicted amino acid residues． Comparison with nucleotide sequence and amino acid sequence homology of ACC oxidase gene ( DQ269492) landed were 99% and 98%，which showed the successful cloning of the ACSI synthase gene．

Key words: Ficus carica, ACC synthase, Gene clone, Sequence analysis

CLC Number:

ZHANG Shuai, ZHANG Ming, KOU Tian-shu, MA Jun-lian, TANG Xia, ZHANG Zi-de. Cloning and Sequence Analysis of a DNA Encoding ACC Synthase Gene from Ficus carica[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 34-37. doi: 10.3969/j.issn.1000-7091.2012.06.008.

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