Cloning a Full cDNA Sequence of FAD8 Gene in Chinese Cabbage

doi:10.3969/j.issn.1000-7091.2012.02.007

Abstract

Abstract: Fatty acid desaturase is a key gene controlled the reaction of linoleic acid to linolenic acid in chloroplast，which affect the membrane fatty acids degree of unsaturation related to plant resistance to cold． A fragment of?DNA，198 bp，of FAD8 gene were cloned in cDNA from cold induced Chinese cabbage leaf depend on the degenerated primers designed by the FAD8 sequence of other plants'． Full cDNA sequence were cloned by rapid amplification of cDNA ends(RACE) PCR，The total cDNA sequeceof FAD8，coding 432 amino acids，is 1 509 bp after sequencing and jointing． Sequence alignment shows high similarity(98%)with FAD8 of Brassica napus． Southern boltting shows it's a single copy gene in Chinese cabbage genome． This work is the basis for further study on chloroplast?fatty acids desaturase．

Key words: Chinese cabbage, Gene of FAD8, Clone

CLC Number:

S642.2
Q78

LIU Chun-xiang, LENG Chuan-yuan, LIU Fu-yan. Cloning a Full cDNA Sequence of FAD8 Gene in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 35-39. doi: 10.3969/j.issn.1000-7091.2012.02.007.

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