Proteomic Analyses of the Early Pm21 Wheat Near Isogenic Lines and Their Parents Affected by Powdery Mildew

doi:10.3969/j.issn.1000-7091.2012.01.002

Abstract

Abstract: The purpose of this project was to study inoculated by E09 in wheat leaves to confer durable and broad spectrum resistance to wheat powdery mildew through proteomic analysis. To carry out the cytological observations of Wheat(Triticum aestivum) cultivars Bainong 3217 and near-isogenic Bainong 3217/Pm21(BC₇F₆) plants were inoculated with E09 powdery mildew after 5 days. Total proteins were extracted by nonionic detergent, were separated by two-dimensional gel electrophoresis(2-DE) 3 replicates and analysed through MALDI-TOF-MS/MS mass spectrometry. Microscopic observation results showed that Bainong 3217 leaves cell surface folds after 12hr, and spores growing significantly inhibited on leave surface of near-isogenic Bainong 3217/Pm21(BC₇F₆) after 2 days inoculation. Besides, hypersensitive reaction was found at 4th day post inoculation. And 15 of up-regulated proteins were identified homologous to known proteins in various databases by MALDI-TOF-MS/MS and MASCOT analysis. The predicted functions of the 5 proteins were related to Chloroplasts photosynthesis(40%), oxygen releasing(13%), Metabolites synthesis(20%), resistance, transcription. One result of leaves deepening green may cause by Enzyme Rubisco and Glutamine synthetase leaf isozyme proteins increased. These proteins increased after about 3 or 4 hours infection. Enzyme Rubisco and Ascorbate peroxidase expressed were up-regulated to protect Calvin cycle in high concentration of H₂O₂. In addition, g lucan endo-1, 3-beta-D-glucosidase precursor induced were up-regulated to suppress further development of haustoria. The potassium channel protein may play an important role in early disease-resistance signal transduction in Pm21.

Key words: Powdery mildew, Near-isogenic lines, Pm21, Microscopy, 2-D electrophoresis, Proteomics

CLC Number:

Q78
S435

LI Da, LI Qiang, GUAN Xi-zhen, ZHANG Wei-dong, TIAN Ji-chun. Proteomic Analyses of the Early Pm21 Wheat Near Isogenic Lines and Their Parents Affected by Powdery Mildew[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 5-11. doi: 10.3969/j.issn.1000-7091.2012.01.002.

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