Development of Fluorescent Quantitative PCR Assay for Porcine SIRT1 Gene

doi:10.7668/hbnxb.2011.S1.009

Abstract

Abstract: SIRT1 gene plays an important regulatory role on cell survival,senescence,apoptosis.This study was to establish a Fluorescent quantitative PCR assay for porcine SIRT1 gene expression.According to the mRNA sequence of SIRT1,primers of porcine SIRT1 gene were designed,and a power SYBR Green Fluorescent quantitative PCR method based on SYBR Green I dye was developed.The results showed that the established Fluorescent quantitative PCR detection method was rapid,sensitive and specific,and so on.The result provided a useful methodological basis for investigating SIRT1 gene.

Key words: Pig SIRT1 gene, Fluorescent quantitative, PCR

CLC Number:

LI Bi-xia, ZHAO Fang, REN Shou-wen, WANG Xue-min, FANG Xiao-min. Development of Fluorescent Quantitative PCR Assay for Porcine SIRT1 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 44-46. doi: 10.7668/hbnxb.2011.S1.009.

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http://www.hbnxb.net/EN/Y2011/V26/IS1/44

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