Molecular Cloning，Expression Character and Bioinformatics Analysis of OsLEA19afrom Rice

doi:10.7668/hbnxb.2011.06.014

Abstract

Abstract: Semi-quantitative RT-PCR analysis revealed that OsLEA19a，a late embryogenesis abundant(LEA) protein gene，was induced by water deficit and salt stress，which sugGested a role for OsLEA19aprotein in water deficit and salt stress protection． The cDNA sequence of OsLEA19awas cloned from rice by RT-PCR． Bioinformatics analysis showed that OsLEA19aencoded a protein of 200 amino acids with a calculated molecular mass of 20． 48 kDa and a theoretical pI of 5． 89． OsLEA19aprotein contains a Pfam: LEA_4 domain architecture at position 5-48， three α-helical domains and without β-sheet domain． In silico predictions showed OsLEA19aprotein was strongly hydrophilic． The phylogenetic relationship between related group 3 LEA proteins from different plants was analyzed， which showed that OsLEA19awas more closely related to group 3 LEA proteins from monocots than from dicots． The amino acid sequence of OsLEA19ashows 48%-57% sequence identity with other members of group 3 LEA proteins from monocots．

Key words: Rice, OsLEA19a, Molecular cloning, Expression character, Bioinformatics

CLC Number:

HU Ting-zhang, WU Ying-mei, CHEN Zai-gang, HUANG Xiao-yun. Molecular Cloning，Expression Character and Bioinformatics Analysis of OsLEA19afrom Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 73-78. doi: 10.7668/hbnxb.2011.06.014.

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