Detection on Cold Tolerance and Analysis on Polymorphic SSR in Oryza rufipogonGriff． Indigenous to Gaozhou，Guangdong

doi:10.7668/hbnxb.2011.06.003

Abstract

Abstract: Cold damage at seedling stage is one of the major factors for reducing the rice products． Gaozhou wild rice(Oryza rufipogonGriff) possesses abundant genetic diversity，and perhaps which carries some strong coldtolerant genes． In this study，cold-tolerant ability was detected at seedling stage in Gaozhou wild rice，and of which two numbers(GZW5 and GZW18) was identified to have strong cold-tolerant ability． Then the polymorphism markers were screened between Gaozhou wild rice with strong cold resistance and its control Sanbaili with cold sensitive by using 225 simple sequence repeat(SSR) markers even distributing on 12 pairs of chromosomes in rice． A total of 83 pairs of SSR markers were identified to have polymorphism，and the polymorphic ratio was 36． 9 percent． These polymorphic markers distributed equally on the chromosomes in rice except the chromosome 5， 10 and 12，which only of 4，3 and 4 polymorphic markers， respectively． The hybrid F₁was constructed between Gaozhou wild rice and Sanbaili， a nd the genotype of its F₂individuals was investigated using some of the polymorphism markers，and in F₂groups these markers generated obviously separation， so we predicted these markers may be linkage with some cold tolerant genes． Our results will provide a basis for mapping the cold-tolerant genes at seedling stage in Gaozhou wild rice and researching further the molecular mechanism of their actions．

Key words: Oryza rufipogonGriff, Gaozhou, Seedling stage, Cold tolerance, SSR markers

CLC Number:

S511． 03

WANG Lan, CAI Dong-chang. Detection on Cold Tolerance and Analysis on Polymorphic SSR in Oryza rufipogonGriff． Indigenous to Gaozhou，Guangdong[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 12-15. doi: 10.7668/hbnxb.2011.06.003.

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