Effects of P1 Infection on the Molecules Associated with Processing and Presentation Function of Porcine Pulmonary Alveolar Macrophages for Exogenous Antigen and Costimulatory Molecules

doi:10.7668/hbnxb.2011.03.007

Abstract

Abstract: The transcriptional levels of SLA-DR,SLA-DM,CD74 and costimulatory molecules CD40,CD80 and CD86 of porcine pulmonary alveolar macrophages(PAMs) on different phases after porcine circovirus-like agent P1 infection were detected by real-time RT-PCR.The results showed that mRNA levels of all above-mentioned molecules were down-regulated at 3 days post-inoculation(DPI).Among those CD80 mRNA expression showed a downward trend(P0.1),and CD40,CD74 and SLA-DR mRNA expression were significantly declined(P0.05).The mRNA expression of SLA-DR SLA-DM at 8 DPI and SLA-DM at 17 DPI were down-regulated(P0.1).CD74 mRNA expression decreased markedly at 40 DPI(P0.05).Whereas the expression levels of CD86 at 24 DPI showed an upward trend(P0.1).These results suggest that P1 infection affected markedly processing and presentation of PAMs for exogenous antigen,which resulting in a decrease and unbalance of antigen presentation function of PAMs for exogenous antigen and impairment for humoral immune responses.

Key words: Porcine circovirus-like agent, Pulmonary alveolar macrophage, Molecules associated with antigen processing and presentation, Costimulatory molecules, Real-time PCR

CLC Number:

WEN Li-bin, HE Kong-wang, YANG Han-chun, LIU Meng-ya. Effects of P1 Infection on the Molecules Associated with Processing and Presentation Function of Porcine Pulmonary Alveolar Macrophages for Exogenous Antigen and Costimulatory Molecules[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 32-36. doi: 10.7668/hbnxb.2011.03.007.

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References

[1] 温立斌,何孔旺,杨汉春.一株类猪圆环病毒2型因子P1的全基因组序列测定与分析[J].中国农业科学,2010,43(2):411-416.

[2] Wen L B,He K W,Yang H C,et al.Complete nucleotide sequence of a novel porcine circovirus-lke agent and its infectivity in vitro[J].Science in China Series C:Life Sciences,2008,51 (5):453-458.

[3] 温立斌,何孔旺,杨汉春.P1因子分子克隆的体外感染性分析[J].畜牧兽医学报,2008,39:941-944.

[4] 温立斌,何孔旺,杨汉春,等.类猪圆环病毒因子P1感染对猪红细胞的影响[J].内蒙古农业科技,2009(2):46-48.

[5] 温立斌,何孔旺,杨汉春,等.TUNEL法检测类猪圆环病毒2型因子P1诱导猪免疫器官细胞凋亡研究[J].华北农学报,2008,23(增刊):88-91.

[6] 温立斌,何孔旺,杨汉春,等.类猪圆环病毒2型因子P1和P2诱导PK-15细胞凋亡的研究[J].华北农学报,2008,23(6):84-86.

[7] Tortorella D,Gewurz B E,Furman M H,et al.Viral subversion of the immune system[J].Annu Rev Immunol,2000,18:861-926.

[8] 温立斌,何孔旺,杨汉春,等.猪抗病毒蛋白基因重组质粒实时荧光定量PCR标准曲线的建立[J].中国农业大学学报,2009,14(2):1-4.

[9] Botner A,Nielsen.J,Bille-Hansen V.Isolation of porcine reproductive and respiratory syndrome (PRRS) virus in a Danish swine herd and experimental inoculation of pregnant gilts with the virus[.J].Vet Microbial,1994,40:351-360.

[10] Koulova L,Clark EA,Shu G,et al.The CD28 ligand B7/BB1 provides costimulatory signal for alloactivation of CD4 + T cell[J].J Exp Med,1991,173:759-762.

[11] Ando A,Kawata H,Murakami T,et al.cDNA cloning and genetic polymorphism of the swine major histocompatibility complex (SLA) class Ⅱ DMA gene[J].Anim Genet,2001,32(2):73-7.

[12] Frauwirth K,Shastri N.Mutation of the invariant chain transmembrane region inhibits Ⅱ degradation,prolongs association with MHC class Ⅱ,and selectively disrupts antigen presentation[J].Cell mmunol,2001,209(2):97-108.

[13] Nakajima A,Kodama T,Morimoto S,et al.Antitumor effect of CD40 ligand:elicitation of local and systemic antitumor responses by IL-12 and B7[J].J Immunol,1998,161(4):1901-1907.

[14] Costello R T,Mallet F,Sainty D,et al.Regulation of CD80/B7-1 and CD86/B7-2 molecule expression in human primary acute myeloid leukemia and their role in allogenic immune recognition[J].Eur J Immunol,1998,28(1):90-103.

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