Molecular Cloning and Characterization of Clga-1 in Cochliobolus lunatus

doi:10.7668/hbnxb.2011.02.009

Abstract

Abstract: Cochliobolus lunatus is a destructive pathogen of foxtail millet and causes significant yield losses.A G protein alpha protein genes named cfga-J(Genbank accession number：HQ69908 1)was cloned using degenerate PCR and RACE method from Cochliobolus lunatus.The predicated C/as-J gene contains a 1062 bp ORF and it was interrupted by three small introns of 57,49,59 bp.The Ctga-J gene encodes a polypeptide of 353 amino acids and phylogenetic analysis showed CtgoL-1 gene was similarity to the Ga subunit of other fungi.The analysis of Southern bloting indicated that there was a single copy of Clgct-j gene in the genome.Clga-Z gene also contained conservative motifs among the Got protein family,such as sites for myristoylation at the N-terminus,ADP-ribosylation by pertussis toxin at the C-terminus.The cloning of the gene will lay afoundation for further functional analysis of heterotrimericG protein signal transduction pathway in Cochliobolus lunatus.

Key words: Cochliobolus lunatus, Heterotrimeric G protein, RACE, Equence analysis

CLC Number:

Q785

LI Zhi-yong, XIE Xia-qing, DONG Jin-gao, DONG Zhi-ping. Molecular Cloning and Characterization of Clga-1 in Cochliobolus lunatus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 39-42. doi: 10.7668/hbnxb.2011.02.009.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2011.02.009

http://www.hbnxb.net/EN/Y2011/V26/I2/39

References

[1] Nicolas R,Darren M S,Cemile A,et al.Comparative genomics of MAP kinase and calcium-calcineurin signaling components in plant and human pathogenic fungi[J].Fungal Genetics and Biology,2009,46:287-298.

[2] McCudden C R,Hains M D,Kimple R J,et al.G-protein signaling:back to the future[J].Cellular and Molecular Life Science,2005,62:551-577.

[3] Horwitz B A,Sharon A,Lu S,et al.A G protein alpha subunit from Cochliobolus heterostrophus involved in mating and appressorium formation[J].Fungal gGenetics and Biology,1999,26:19-32.

[4] Rahim M,Sarrah B M,Theo A J,et al.Ga and Gβ proteins regulate the cyclic AMP Pathway that is required for development and pathogenicity of the phytopathogen Mycosphaerella graminicola[J].Eukaryotic Cell,2009,8(7):1001-1013.

[5] Peter S S,Kar C T,Pedro S,et al.The disruption of a Ga subunit sheds new light on the pathogenicity of Stagon-osporu nodorum on wheat[J].Molecular Plant-microbe Interactions,2004,17 (5):456-466.

[6] Wang N Y,Lin C H,Chung K R.A Ga subunit gene is essential for conidiation and potassium efflux but dispensable for pathogenicity of Alternaria alteruata on citrus[J].Current Genetics,2010,56:43-51.

[7] Hua C L,Wang Y L,Zheng X B,et al.A Phytophthora sojae G-protein at subunit is involved in chemotaxis to soybean isoflavones[J].Eukaryotic Cell,2008,7 (12):2133-2140.

[8] Simon M I,Strathmann M P,Gautam N.Diversity of G proteins in signal transduction[J].Science,1991,252:802-808.

[9] Julia S,Muriel V,Adeline S,et al.The Ga subunit BCG1,the phospholipase C (BcPLC1)and the calcineurin phosphatase co-ordinately regulate gene expression in the grey mould fungus Botrytis cinerea[J].Molecular Microbiology,2008,67 (5):1027-1050.

[10] Field T A,Casey P J.Signaling functions and biochemicai properties of pertussis toxin-resistant G-proteins[J].Biochem,1997,321:561-571.

[11] Maria J A,Amorim J,Bruno S D,et al.Functional and genetic characterization of calmodulin from the dimorphic and pathogenic fungus Paracoccidioides brasiliensis[J].Fungal Genetics and Biology,2003,39:204 -210.

[1]	SHI Yumei, CHEN Shaokang, XING Kai, ZHAO Yanhui, YUAN Jiani, SHENG Xihui, QI Xiaolong, NI Hemin, GUO Yong, WANG Chuduan. Identification of Differential Genes of Longissimus Dorsi Muscle of Songliao Black Pig and Landrace Pig Based on High-throughput Sequencing Technology [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 222-229.
[2]	XIAO Shikui, LI Fang, ZHANG Wenting, LÜ Shufang, SHI Guoan, WU Jiang, FAN Bingyou. *Cloning and Prokaryotic Expression of ACS* Gene from Paeonia lactiflora Pall.** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 36-44.
[3]	LIAN Kaiqi, JI Shuang, ZHOU Lingling, SHI Zhiqi, WANG Fei, ZHANG Yuanchen. Cloning and Prokaryotic Expression of a clip-type Serine Protease Gene from Mythimna separate [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 195-201.
[4]	JIN Fengmei, XUE Jun, SUN Haibo, HAO Zhiyu. *Molecular Detection and Partial Genomic Sequence Analysis of Tomato mosaic virus* in Tianjin Based on Small RNA Sequencing** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 176-183.
[5]	AERZUGULI Maimaitituerxun, ZHANG Zijun, ZHANG Weihong. *Complete Mitochondrial Genome Sequence of Oxyloma wujiaquensis* Li, Guo & Zhang, 2017(Gastropoda：Succineidae)** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 230-238.
[6]	HU Yueqing, WANG Ruozhong, HUANG Zhigang, XIAO Langtao. *Cloning and Prokaryotic Expression of Transcription Factor StWRKY57* Gene in Potato** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 10-17.
[7]	HE Shipeng, LIANG Bin, WU Dejun, WANG Qunyan, LI Junliang. Nutrient Demand of Tomato in the Facility Vegetable Field [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 282-288.
[8]	WANG Fei, ZHONG Xionghui, CHEN Denghui, LI Hailong, KANG Jungen, XIE Jianming. *Optimization of Protoplasts Preparation System and Establishment of Transient Transformation System in Brassica oleracea* var. capitata L.** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 69-78.
[9]	LIU Kunang, HUANG Yali, JIA Zhenhua, MA Hong, SONG Shuishan. Screening and Identification of Bacillus sp. and Its Metabolites Inhibitory Effect Against Botrytis cinerea [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 200-207.
[10]	ZHANG Xi, QIAN Yuyuan, LIU Yi, WANG Guang, SONG Shijia, LI Xiaofei, MI Huanfang, CUI Shufang, LI Junlan. Cloning and Expression Analysis of a Cotton Pigment Gland Formation Related Gene GhNAC201 [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 29-35.
[11]	ZHEN Junbo, LIU Di, SONG Shijia, LIU Linlin, CAI Xiao, ZHANG Xi, LI Haishan, CHI Ji. Cloning and Expression Analysis of Two Late Embryogenesis Abundant(LEA) Proteins from Upland Cotton [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 33-38.
[12]	JIA Mengzhen, ZHANG Bin, TIAN Miao, JIE Yan, ZHAO Yuqi, GAO Xinying, GU Xin, DING Junjie, CHEN Yufei, WEN Jingzhi. *Spatial-temporal Dynamics of Physiological Races and Virulence Structure of Phytophthora sojae* Population in Eastern Heilongjiang Province** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 208-215.
[13]	LIU Yufeng, ZHU Tianhui, LIU Yinggao, QIAO Tianmin, LI Shujiang, LONG Xumei, HAN Shan. *Cloning, Sequence Analysis and Prokaryotic Expression of CmWRKY* from Castanea mollissima BL** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 37-45.
[14]	SONG Jian, XUE Jun, JIN Fengmei, SUN Haibo, ZHANG Yue, WANG Shu, FAN Huan, ZHOU Xiangming, CHEN Rui. Partial Virus Gene Sequence and Real-time Quantitative PCR Analysis of Tomato after Mixed Infection of ToCV and TYLCV [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 95-102.
[15]	WEN Juanjuan, WANG Chaonan, HUA Deping, ZHANG Hong, XU Yingli, ZHANG Bin. *Reaction and Resistant Gene Loci Detection of Different Chinese Cabbage Varieties to Different Plasmodiophora brassicae* Strains** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 103-109.

Please choose a citation manager

Content to export