Abstract: To construction and identification of the expression plasnid containing genes encoding Easy gene of Myrobacteriun trrberculosis and the large envelope protein L of HBV,and trarrsfoan the recanb inapt vec br inb Agrobacteriun trxnefaciens LBA4404. The L and East6 gene were amplied form pPIC9K-L, and Mycobacterixn trrberculosis genome by PCR, then the fusion DNA fragnent of L and East6 were applied by Splicing by Overlap Extension were cloned into the vector pEGG. The combine fragrrent of prcmoterglobulin-l and the targetgene L-East6 which get film doubled enzymes digestion of the recombined plasmid pEG-G-L-Esat6 was inserted into the plant expression vector pCAMB IA1300 which contain the gene bar for herbicide resistance. Then transfomed recanb inapt plasin id pCAM-G-L-Esat6 into Agrobacteriun tumerfaciens LBA4404. We have successfuliy constructed eukaryotic expression recombinantp lasmid pCAMG-L-Esat6 and it is shoved that the cloned sequence of L and Esat6 is correct by sequencing.We have successfully constructed expression plasmid containing genes encoding L protein of HBV and Esat6 gene and transfomed into LBA4404, and lays a foundation for construction combined gene vaccine against HBV and MTB.

Key words: Mycobacterium habereulosis, HBV, L gene, Esat6, globulin-l

CLC Number: 

• Q786