Construction of Plant Expression Vector on Fusion Transit Peptide ofCAC3Gene andaccDGene

doi:10.7668/hbnxb.2008.05.006

Abstract

Abstract: The acety-l CoA carboxylase(ACCase)is the rate-limiting enzyme of fatty acids synthesis TheaccDgene and transit peptide ofCAC3gene was amplified fromGossyiuum hirsutumgenome andArabidopsis thalianagenome,DNA sequence analysis indicated that the cloned DNA fragmes have the highest homology compared with the NCBI published date,showing 100% and 99%.Plant expression vector of fusion transit peptide ofCAC3gene andaccDgene was con- structed The vector ofpBI-CAC3tp-accDwill be usefull for further natural genetic transformation.

Key words: Gossypium hirsutum, Arabidopsis thaliana, accDgene, CAC3gene, Transit peptide

CLC Number:

Q786

ZHANG Yu-xing, CUI Yan, WU Han-xue, ZHU Jian-bo, ZHOU Peng. Construction of Plant Expression Vector on Fusion Transit Peptide ofCAC3Gene andaccDGene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 26-29. doi: 10.7668/hbnxb.2008.05.006.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.05.006

http://www.hbnxb.net/EN/Y2008/V23/I5/26

References

[1] 石东乔,周奕华,陈正华.植物脂肪酸调控基因工程研究[J].生命科学,2002,14(5):291-295.
[2] 王幼平,曾宇,罗鹏.植物脂肪酸代谢工程研究进展[J].中国油料作物学报,1998,20(4):88-92.
[3] 张羽航,林炜铁,姚汝华.植物油脂基因工程[J].中国油脂,1999,24(5):56-58.
[4] Jins han Ke,Tuan-Nan Wen.Coordinate regulation of the nuclear and plastidic genes coding for the subunits of the heteromeric acetyl-coenzyme A carboxylase1[J].Plant Physiology,2000,122:1057-1071.
[5] Sang Sook Lee,Won Joong Jeong.Characterization of the plastid-encoded carboxyltransferase subunit(accD)gene of potato[J].Mol Cell,l17,3:422-429.
[6] Norihiro Hirata,Daizou Yonekura.Possible involvement of the 5'-flanking region and the 5'UTR of plastid accD gene in NEP-dependent transcription[J].Plant Cell Physiol,2004,45(2):176-186.
[7] 王伏林.植物中的乙酰辅酶A羧化酶[J].植物生理学通讯,2006,42(1):10-14.
[8] 赵虎基.植物乙酰辅酶A羧化酶的分子生物学与基因工程[J].中国生物工程杂志,2003,23(2):12-16.
[9] 谢禄山,谭晓风.乙酰辅酶A羧化酶基因研究综述[J].中南林学院学报,2005,25(4):89-95.
[10] Yukiko Sasaki,Yukio Nagano.Plant Acetyl-CoA carboxylase:structure,biosy-nthesis,regulation,and gene manipulation for plant breeding Biosci[J].Biotechnol Biochem,2004,68(6):1175-1184.
[11] Yukiko Sasaki,Kazuhiko Hakamada.Chloroplast-encoded protein as a subunit of acetyl-CoA carboxylase in pea plant[J].Biological Chemistry,1993,268(25):25118-25123.
[12] Yuka Madoka,Ken-Ichi Tomizawa.Chloroplast transformation with modified accD operon increases acetyl-CoA carboxylase and causes extension of leaf longevity and increase in seed yield in tobacco[J].Plant Cell Physiol,2002,43(12):1518-1525.
[13] 马三梅,王永飞.高等植物叶绿体基因组的转化[J].植物生理学通讯,2004,40(3):385-389.
[14] 卢善发.植物脂肪酸的生物合成与基因工程[J].植物学通报,2000,17(6):481-491.
[15] Dorte Klaus,John B.Ohlrogge.Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase[J].Planta,2004,219:389-396.
[16] 萨姆布鲁克.分子克隆实验指南[M].黄培堂译.北京:科学出版社,2002.

Please choose a citation manager

Content to export