Study on Virus Replicase Gene Mediated Maize Resistance to MDMV

doi:10.7668/hbnxb.2008.05.025

Abstract

Abstract: Through pollen-mediate's method,virus replicase gene was transformed into maize lines.These transgenic maize seedings in 5-6 leaf stages were mechanically inoculated with severestrains of maize dwarf mosaic virus(MD-MV).They mostly showed a high level resistant to MDMV infection.The results of PCR and PCR-Southern Blot analysis had conformed that target gene was integrated into the genome of T₁and T₂transgenic maize.Transgenic maize lines strongly resistant to the disease were obtained through MDMV field test of T₁and T₂.

Key words: Virus replicase gene, Maize, Pollen-mediate's method, Artificial inoculation, MDMV

CLC Number:

S513.03

LEI Hai-ying,SUN Yi, WANG Zhi-jun, DU Jian-zhong. Study on Virus Replicase Gene Mediated Maize Resistance to MDMV[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 114-117. doi: 10.7668/hbnxb.2008.05.025.

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