A Method of Rapid and Efficient Isolation of Highly Specific Antibodies from an Antiserum Against a Pool of Proteins

doi:10.7668/hbnxb.2008.05.024

Abstract

Abstract: The common method for monoclonal antibody preparation is complex and time-consuming.So a procedure which allowed rapid and efficient isolation of target protein from a library of antibodies was reported here.According to immunology protocol,specific antibodies to a peritrophic membrane protein,SeP89(which was expressed in Escherichia coli),was isolated from a polycolonalantiserum against a pool of proteins from the PM of Spodoptera exigua.We successfully isolated highly specific antibodies to SeP89 protein from a polycolonal antiserum pool.A method of rapid and efficient isolation of highly specific antibodies from an antiserum against a pool of proteins was established.

Key words: Antibody, cDNA clone, PM protein, Antiserum, Western blot

CLC Number:

Q511

ZHANG Xia, LI Jie, GUO Wei, LI Guo-xun,. A Method of Rapid and Efficient Isolation of Highly Specific Antibodies from an Antiserum Against a Pool of Proteins[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 111-113. doi: 10.7668/hbnxb.2008.05.024.

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http://www.hbnxb.net/EN/Y2008/V23/I5/111

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