Induction and Rapid Propagation of Dioscorea nipponica Makino′Root and Mensuration of Diosgenin′Content

doi:10.7668/hbnxb.2007.S1.046

Abstract

Abstract: Induction and rapid propagation of Dioscorea nipponica , China root were studied using clustered shoots asmaterial ,meanwhile , contents of diosgenin in roots were determined by RP-HPLC , the results as follows:1/2 MS mediumwith no hormones was furthest suitable for roots induction with induction rate of 76.7 %;the best medium for root′propagationisMS +0.2 mg/L NAA +0.5mg/L IBA +0.2 -0.5mg/L PP333 , and the proliferation time was more than 93 after 60days;root′growth form changed during the course of rapid propagation , difference of diosgenin′content in different rootswasn′t notability , and the average is 0.21 %.

Key words: Dioscorea nipponica Makino, Root, Tissue culture, Diosgenin

CLC Number:

S567.239

JIANGYu-bao, ZHANGSu-mei, YUYuan-jie. Induction and Rapid Propagation of Dioscorea nipponica Makino′Root and Mensuration of Diosgenin′Content[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.046.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2007.S1.046

http://www.hbnxb.net/EN/Y2007/V22/IS1/189

[1]	BAO Xiuxia, LIAN Yong, MU Zongjie, ZHANG Huan. *Comparative Study on Root and Leaf Photosynthesis of Allium polyrhizum* from Different Sources under Drought Stress** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 122-127.
[2]	HU Jinge, BAI Shijian, PAN Xubing, CHEN Guang, ZHAO Ronghua, CAI Junshe. Effects of Different Rootstocks on Wine Quality in Wine Grapes Marselan [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 141-150.
[3]	JIA Jianping, LI Jinhong, YU Jingwen, YU Xiyue, PENG Deliang, LI Huixia, HUANG Wenkun. Effect of Nitrogen Fertilizer on Soybean Cyst Nematode Disease [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 198-205.
[4]	LI Wu, LI Chunyan, LI Guangyu, LIANG Minyi, LI Yuliang, WEN Tianxiang, TU Panfeng, LIU Jianhua. Effects of Root Application of γ-aminobutyric Acid on Plant Growth, Photosynthetic Characteristics,Antioxidant Physiology and Yield of Waxy Corn [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 72-80.
[5]	GAO Yanlong, ZHANG De, ZHANG Rui, ZHANG Zhongxing, ZHAO Ting, WANG Shuangcheng, WANG Yanxiu. Effect of Exogenous CaCl₂ on Physiological Characteristics of Apple Rootstock T337 under Salt Stress [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 118-126.
[6]	ZHONG Tingting, GUO Shifen, LU Wenbin, XIAO Gang. *Development and Utilization of KASP Marker for Brassica napus* Clubroot Resistance Breeding** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 184-190.
[7]	ZHANG Xiangyu, YU Meixia, TAN Jingai, WANG Peng, LI Caijing, WU Guangliang, CHENG Qin, WANG Yanning, JIANG Ningfei, HUANG Shiying, HE Haohua, BIAN Jianmin. Mapping QTLs Tolerant to Cold Stress in Rice Root by Chromosome Segment Substitution System [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 83-89.
[8]	LI Jingyi, LIANG Yugang, WANG Ren, MENG Xiangjie, GONG Xiangsheng, HUANG Huang. Effects on Root Growth Characteristics in No-tillage Semi-solid Direct Seeding of Rice [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 135-145.
[9]	GAO Zhihong, LIN Yuxia, ZHANG Yupeng, LIANG Jiali, LUO Wenjun, CHEN Xiaoyuan. Growth and Ion Content in Rice Seedling Xylem Sap under Different Water Stresses and Nitrogen Forms [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 146-153.
[10]	HUANG Chenchen, SONG Xiao, HUANG Shaomin, ZHANG Keke, YUE Ke. Analysis of Differences in Root Morphology, Phosphorus Transport and Yield of Wheat with Different Phosphorus Efficiency [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 169-175.
[11]	CUI Baowei, LIU Quanyong, QIN Guangli, ZHU Junpeng. Effect of Combined Application of Fermented Sheep Manure and Soil Loosening and Root Promoting Agent on Soil Physical and Chemical Properties Yield and Quality of Red Cluster Pepper [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 182-187.
[12]	NIU Ruimin, HUANG Xiaojing, SHEN Tian, XU Zehua, CHEN Weiping. Effects of Different Rootstock on the Growth, Yield and Fruit Quality of Chardonnay and Merlot Grapes [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 116-123.
[13]	WANG Yanhui, SUN Haowei, WEI Chaohui, WANG Li. Effects of Activated Water on Growth and Root Activity of Wheat [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 124-133.
[14]	PENG Guangjue, WANG Zhiyong, HU Tong, ZHENG Zhongyi, TANG Chungui, LIU Yongjun, LI Qiang, ZHOU Qingming, LI Juan. Effects of Deep Vertical Rotary Tillage on Soil Physical Characteristics and Root Development of Flue-Cured Tobacco in Paddy-Tobacco Growing Area in Changsha [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 134-142.
[15]	LI Shengyou, CAO Yongqiang, WANG Changling, ZHANG Lijun, WU Lishi, ZHANG Li, CHEN Yanqiu, WANG Wenbin, SONG Shuhong. Evaluation of Contribution of Different Genotypic Roots to Grain Yield in Soybean by a Grafting Experiment [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 161-166.

Please choose a citation manager

Content to export