Growth and Ion Content in Rice Seedling Xylem Sap under Different Water Stresses and Nitrogen Forms

doi:10.7668/hbnxb.20191601

Abstract

Abstract: To reveal the response mechanism of rice to water stress based on the regulation of xylem flow ions, a whole root and split root hydroponic experiment simulated by PEG was conducted using rice variety Hengfengyouhuazhan as material.The trials had three water conditions:non-water stress(-PEG),partial root drying(PRD),and all root water stress(+PEG),and three levels of nitrogen forms:NH₄⁺-N/NO₃^--N of 50/50,100/0, 0/100,and dry weight,content of chlorophyll and xylem sap ion of rice were determined.The results showed that the dry weight of+PEG plants declined remarkably compared to-PEG plants after 5 days treatment with water stress and nitrogen forms, in which, 0/100 treatment had the biggest drop by 18.09%. However, under PRD condition, the dry weight of plants decreased relatively little, and 100/0 treatment decreased only by 3.72%. Under different water conditions, the chlorophyll content of-PEG condition plants was the largest, and that of+PEG condition plants was the smallest, Under three water conditions, 100/0 treatment had the largest chlorophyll content, and 0/100 treatment had the smallest,among them, treatment 5 was 1.66 times of treatment 6, treatment 8 was 2.42 times of treatment 9. After 5 days of treatment, the contents of K⁺ and Ca²⁺ in xylem sap of all treatments were increased, and the treatments under water stress were more than those under non water stress conditions. However, the K⁺ content was the highest in+PEG treatment, treatment 9 was 5.48% higher than treatment 3,while the Ca²⁺ content was the highest in PRD treatment, treatment 4 increased by 7.04% compared with treatment 1. Under the same water condition, there was no significant difference in the contents of K⁺ and Ca²⁺ among different nitrogen forms. After 5 days of treatment, the water-soluble phosphorus content in xylem sap of all treatments decreased, but the decrease was not significant in-PEG treatment, significant in+PEG and PRD treatment, among which+PEG treatment decreased by 14.6% on average, under both+PEG and PRD water stress conditions, 50/50 treatment had the smallest reduction, and the 0/100 treatment had the largest reduction. These results indicated that, K⁺ and Ca²⁺ in xylem sap were involved in rice water stress response.

Key words: Rice, Partial root drying, Nitrogen form, Xylem sap ion content

CLC Number:

S143.1

GAO Zhihong, LIN Yuxia, ZHANG Yupeng, LIANG Jiali, LUO Wenjun, CHEN Xiaoyuan. Growth and Ion Content in Rice Seedling Xylem Sap under Different Water Stresses and Nitrogen Forms[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 146-153. doi: 10.7668/hbnxb.20191601.

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