Study on Culturing Condition of Glucanases Produced by Coniothyrium minitans

doi:10.7668/hbnxb.2007.S1.045

Abstract

Abstract: In this study through the different carbon source , the nitrogen source and the double carbon source are mixed in the SMCS nutrient fluid .The culturing time , the temperature and the pH are changed in order to study culturing condition of glucanases produced by Coniothyrium minitans .The result indicated that , Coniothyrium minitans on the improved SMCS nutrient fluid at the constant temperature on the skakier(200 r/min ,20 ℃)about 15 d ,may induce produces massive glucanases .The improved SMCS nutrient fluid formula is :KH₂PO₄ 680 mg ,KH₂PO₄ 870mg ,KCl 200mg ,NH₄NO₃ 1 g , MgSO₄·7H₂O 200mg ,CaCl₂ 200mg , FeSO₄ 2 mg , ZnSO₄ 2mg ,MnSO₄ 2mg , saccharose 10 g ,water 1 000mL , adjusting the pH to 6.5

Key words: Coniothyrium minitans, Glucanases, Culturing condition

CLC Number:

S476

LIXin, GAOJun-ming, MALi-na. Study on Culturing Condition of Glucanases Produced by Coniothyrium minitans[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.045.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2007.S1.045

http://www.hbnxb.net/EN/Y2007/V22/IS1/185

[1]	SHI juan, LI Yongli, CHANG Le, ZHENG Weifan, ZHOU Zhou, QU Liangjian. *Analysis of the Growth Promoting Effect and Mechanism of Streptomyces costaricanus* A-m1** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 205-211.
[2]	TAN Ying-hui, YU Bo, LI Zhi-yong, SUN Zhi-ying, LIU Ning, SHEN Shen, CAO Zhi-yan, HAO Zhi-min, DONG Jin-gao. Isolation and Identification of an Antagonistic Bacterial Strain YM-8 Against Fusarium proliferatum [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 287-291.
[3]	SONG Jian, CAO Wei-ping, ZHANG Hai-jian, WANG guang-jun, DU Li-xin. *The HFW-05 Strains of Beauveria bassiana* in Persistent Study on Vegetables** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 336-341.
[4]	WANG Yong, GAO Wei, ZHANG Chun-xiang, HUO Jian-fei, LI Jian-qiang, LUO Lai-xin. *Dynamic Changes of Extra-cellular Enzyme Activity and Reducing Sugar Consistency During the Submerged Culture of a Tirchoderma viride* Isolate(Tr9701)** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 226-230.
[5]	LU Cai-ge, ZHANG Dian-peng, LIU Wei-cheng, WU Hui-ling, LIU Ting, LIU De-wen. Screening,Identification and Activity Analysis of Antagonistic Bacteria against Cabbage Wilt Disease [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 195-202.
[6]	ZHANG Cui-mian, JIA Nan, HU Dong, WANG Zhan-wu. Studies on Fermentation Conditions of Solid Feed with Composite Probiotics [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(S1): 270-274.
[7]	TIAN Zhao-feng, LIU Ting, ZHANG Tao-tao, LI Yong-dan, DONG Dan, LIU Wei-cheng. Strain Identification of Actinomycetes Yn168 and Influence of Its Fermentation Condition on Antiviral Activity [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 204-208.
[8]	CAO Wei-ping, SONG Jian, LU Qing, WEI Li-min, FENG Shu-liang,DU Li-xin. Effects of Chemical Pesticides on Entomopathogenic Fungus Beauveria bassiana HFW-05 Strain and the Joint Toxicity of Their Mixtures against the Diamondback Moth， Plutella xylostella( L． ) ( Lepidoptera: Plutellidae) [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(S1): 358-362.
[9]	SONG Jian, CAO Wei-ping, LU Qing, DU Li-xin. *Sensitivity to Changes of Environmental Factors on Beauveria bassiana* HFW-05 Strain** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(S1): 363-367.
[10]	SONG Jian, CAO Wei-ping, ZHEN Wei, CHEN Dan, DU Li-xin. Preliminary Study of the Classification， Identification and Biological Characteristics of Four Beauveria bassiana [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(S1): 374-376.
[11]	SONG Jian, CAO Wei-ping, DU Li-xin, WANG Gang, WANG Rong-yan, WANG Jin-yao, FENG Shu-liang. Virulence Determination of Beauveria bassiana HFW-05 Strain on Parasitoid Insects [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S2): 180-183.
[12]	LU Cai-ge, PAN Zheng-yan, LIU Wei-cheng, SUI Qin, QIU Ji-yan. The Characteristics for Separation and Identification of the Bioactive Metabolite of Antagonistic Actinomyces A02 [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 147-152.
[13]	DU Li-xin, DONG Ming, WANG Rong-yan, MA Ming-ze, WANG Jin-yao, CAO Wei-ping, SONG Jian, FENG Shu-liang. *Identification of cry Gene from a Novel Bacillus thuringiensis* Isolate SHJ-5** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 194-197.
[14]	SONG Ping, GUO Li-wei, SU Jun-ping, WANG Qin-ying. Studies about Bacillus thuringiensis Strain Containing New cry Gene [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 54-57.
[15]	SONG Ying, WANG Chao-zhong, GUO Shi-ying. Isolation and Antiviral Effect of the Strain Actinomyces Ys.03 [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 164-166.

Please choose a citation manager

Content to export