Abstract: In order to improve the rate of fermentation and exploit Trichoderma Tr9701,the effect of liquid culture on different extracellular enzyme activity and the dynamic changes of reducing sugar content were studied.The results showed that at 25 ℃,110 r/min thermostatic culture conditions,biomass of green Trichoderma hyphae reached the peak in the 6-10 d;the pH value of the fermentation liquid occurs in the first 6-10 d turning gradually increased,then remained stable;extracellular proteins,extracellular sugar reached peak at 6,12 d,the secretion of guaiacol oxidase and polyphenol oxidase with a periodic curve of 5-6 d presents the enhancement curve type.The extracellular amylase,extracellular CMCase activity increased significantly in the early culture period,and the activity of extracellular CMCase was significantly higher than that of amylase and sucrose invertase in liquid fermentation.For realizing the scale fermentation of Trichoderma viride Tr9701,we should be determined the fermentation process according to the changes of extra-cellular enzyme activity and reducing sugar consistency during the Submerged culture of Tirchoderma viride.

Key words: Tirchoderma viride, Submerged culture, Reducing sugar, AmyLase, CMCase, Guaiccal oxidase

CLC Number: 

• S476+.8