Cloning and Sequence Analysis of the PhyB Gene of Aspergillus niger WP1

doi:10.3321/j.issn:1000-7091.2007.02.004

Abstract

Abstract: The PhyB₁ encoding phytase of Aspergillus niger WP1 was amplified by the polymerase Chain reaction (PCR) with primers designed according to the sequences in GeneBank. The amplified fragment was cloned and sequenced. The results showed that the coding region was 1560 bp in size, includes 3 introns, and encoded a peptide of 460 amino acid residues. Comparison of this sequence with the Aph of the nature A. niger ALKO243 (GeneBank Accession: L02420), shows that the nucleotide homology is 96.79%, and the amino acid homology comes up to 99.13%. The PhyB₁ ofA. niger WP1 strain in this paper its sequence has been accessed by GeneBank (Accession: DQ836360).

Key words: Aspergillus niger, PhyB gene, PCR, Sequence analysis

CLC Number:

Q789

KE Xiao-jing, GUO Run-fang, YU Hong-wei, JIA Ying-min. Cloning and Sequence Analysis of the PhyB Gene of Aspergillus niger WP1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 14-17. doi: 10.3321/j.issn:1000-7091.2007.02.004.

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