Relationship Between the Outer Surfaces of Plant Cells Cultured in vitro and the Frequencies of Transformation Agrobacterium-mediated

doi:10.3321/j.issn:1000-7091.2001.03.005

Abstract

Abstract: The outer surfaces of plant cells from tobacco (Nicotiana tabacum),maize( Zea mays ),and rye( Secale cereale )cultured in vitro have been studied using scanning electron microscopy(SEM).The outer surfaces of cell wall(OSCW) of the cells from tobacco subcultures are smooth,and most of the cells are collapsed or crinkled after specimen prepared for SEM.In contrast,there are many protuberances or beads and/or fibres or meshwork on OSCW of the tobacco cells treated for Agrobacterium mediated transformation.Bacteria crowd on the granulated surfaces of the tobacco cells,but little on the smooth surfaces.The same as tobacco cells,OSCW are granulated for the cells from pale yellow,soft and friable and fast grown embryogenic calluses of rye and from the pale yellow calluses of maize.OSCW are smooth for the cells from white and soft calluses of maize,and only a few cells haven't been collapsed in these calluses.The relationship between Agrobacterium attachment sites,which could be induced,on the cell wall surfaces,and the frequencies of genetic transformation mediated by Agrobacterium were discussed.

Key words: Culture in vitro, Outer surfaces of cell wall, Agrobacterium-mediated, Frequencies of transformation

CLC Number:

S188

GUO Xiaocai. Relationship Between the Outer Surfaces of Plant Cells Cultured in vitro and the Frequencies of Transformation Agrobacterium-mediated[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(3): 23-27. doi: 10.3321/j.issn:1000-7091.2001.03.005.

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