Abstract:
Plants were regenerated with high frequency from immature tassels of Zea mays L.(maize inbred 330),Zea diplopetennis,and Zea maysZea×Zea diplopennis hybrid cutured in vitro.Adventitious buds and shoots were directly developed in the primary culture from the immature tassels cultured on the medium MS containing 2 mg/1 6-BA.Subcultured on the same medium,rapid,clonal bud and shoot propagation was obtained.The buds and shoots were very easy of rooting by transferring them to root-inducing medium,MS containing 0.5 mg/1 NAA,300 mg/1 lactalbumin hydrolyzate(LH),and 1000 mg/1 activated charcoal.The developmental stage of immature tassels and the combination of phytohormones were most important for successful culture establishment.The application of this technique was discussed.
Key words:
Zea diploperennis,
Immature tassel culture in vitro,
Direa formation of adventitious buds and shoots
Guo Xiaocai, Zhang Yuling. In Vitro Culture of Immature Tassels of Zea diploperemis and Its Interspecific Hybrid.Ⅰ.Direct Formation of Adventitious Buds and Plant Regeneration[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1988, 3(4): 16-22. doi: 10.3321/j.issn:1000-7091.1988.04.004.