Transformation of Engineered Male Sterile Gene and Establishment of Transgenic Plants in Sesame (Sesamum indicum L.)

doi:10.3321/j.issn:1000-7091.1996.04.007

Abstract

Abstract: The engineered male sterile gene barnase together with the mark gene bar were transformed to the isolated cotyledon of a sesane variety Yuzhi 4 by microprojectile bomebardment.The transformed cotyledon was cultured under darkness for 12 days then converted into dark 1ight alternative conditions on the culture media MS+5mg.L ^-1 6-BA+1mg•L^-1 IAA+1mg•L^-1 ABA+5mg•L - AgNO₃ Resistant calli were selected on herbicide Basta 2mg.L^-1 11 anti Basta plants were obtained.Southern blot of 3 well developed green plants confirmed stable integration of both barnase and bar genes into nuclear DNA.The transfomation efficiency reached 1.46%.

Key words: Sesame, Microprojectile bombardment, Cotyledon, Barnase gene, Bar gene, Transgenic sesame plant

CLC Number:

S565.3

Chen Zhankuan, Zhi Yubao, Yi Minglin, Wang Jinlan, Liang Xiuyin, Tu Lichuan, Fu Rongzhao, Cao Guangcheng, Shi Yanhong, Sun Yongru. Transformation of Engineered Male Sterile Gene and Establishment of Transgenic Plants in Sesame (Sesamum indicum L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1996, 11(4): 33-38. doi: 10.3321/j.issn:1000-7091.1996.04.007.

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