Plant Regeneration via Tissue Culture of Garlic Pedicel

doi:10.3321/j.issn:1000-7091.1992.03.013

Abstract

Abstract: Calli appeared from pedicels of garlic cultured on N8+2,4-D2mg/L+KTlmg/L for 30 days, These calli survived two subcultures on N8+2,4-D 0,5mg/L+KT 0.5mg/L,Organogenesis occurred on NB+BA 5mg/L+KT lmg/L+IAA O,Olmg/L MS(令Macroelements)+BA 5mg/L+KT 1mg/L+IAA O.Olmg/L and MS+BA 5mg/L+KT lmg/L+IAA 0.01mg/L, Roots appeared one week after the shoot was transferred onto the MS(专Macroelements)+NAA O.Olmg/L medium2,4-D and KT were essential to the induction of calli from garlic pedicels, Higher concentrations of plant growth regulators，i.e,cytokinin/auxin, lower concentration of NH李and cold treatment to calli were all essential to shoot formation, Cellular observation on the process of callus induction from garlic pedicel is also, disscussed.

Key words: Garlic, Pedicel, Plant regeneration, Plant growth regulators

Wang Honglong, Kang Yuqing, Zhang Cunjin, Ma Yun, Wang Taokuan. Plant Regeneration via Tissue Culture of Garlic Pedicel[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1992, 7(3): 66-70. doi: 10.3321/j.issn:1000-7091.1992.03.013.

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