Effects of Different Plant Growth Regulators(PGRs)on Protective Enzyme Activity and Metabolism Assimilation in Soybean Leaves

doi:10.7668/hbnxb.2008.03.026

Abstract

Abstract: The aim of this experiment was to discover difference of protective enzyme activity and study effects of metabolism assimilation in soybean leaves by spraying different plant growth regulators(PGRs).It was also to provide references for anti-aging and increasing function of metabolism assimilation in the pod filling(R5)stage.Field experiments was conducted the effects of different PGRs on metabolism assimilation and activity of protective enzyme,and Kennong4 variety was selected to avoid the impact of genetic factors in 2006.The results showed that three regulators(SOD simulation material(SODM),Choline chloride(Cc)and Diethyl aminoethyl hexanoate(DTA-6)increased SOD and POD activity with a continuation of spraying time in soybean leaves.SOD activity of DTA-6 was the highest,followed by SODM;POD activity of SODM was the highest,next came Cc.In addition,SODM and DTA-6 also increased the CAT activity of leaves at a certain extent,enhanced soluble proteins,soluble sugar,as well as to nitrate content of free amino acids,and slowed down the increase of MDA.But Cc was not obvious.The above results indicated that it is effective to enhance the antioxidant capacity and slow down the senescence of its leaves,and maintaining physiological assimilation function of its metabolism assimilation and activity of protective enzyme in soybean leaves.

Key words: Plant growth regulators, Soybean, Protective enzyme, Metabolism assimilation

CLC Number:

S55.1

ZHAO Li-ming, ZHENG Dian-feng. Effects of Different Plant Growth Regulators(PGRs)on Protective Enzyme Activity and Metabolism Assimilation in Soybean Leaves[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 106-111. doi: 10.7668/hbnxb.2008.03.026.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.03.026

http://www.hbnxb.net/EN/Y2008/V23/I3/106

References

[1] Che F S,Cho C,Hyeon S B,et al.Metabolism of choline chloride and its analogs in wheat seed-lings[J].Plant Cell Physiol,1990,31:45-50.

[2] Uemura M,Joseph R A,Steponkus P L.Cold acclimation of Arabidopsis thaliana[J].Plant Physiol,1995,109:15-30.

[3] Uemura M,Steponkus P L.A contrast of the plasma mem-brane lipid composition of oat and rye leaves in relation to freezing tolerance[J].Plant Physiol,1994,104:479-496.

[4] 盛瑞艳,李鹏民,薛国希,等.氯化胆碱对低温弱光下黄瓜幼苗叶片细胞膜和光合机构的保护作用[J].植物生理与分子生物学学报,2006,32(1):87-93.

[5] 张燕,方力,李天飞,等.氯化胆碱浸种对烟草幼苗某些生理特性的影响[J].植物生理学通讯,2004,40(2):164-166.

[6] 祁春苗,张秀月,赵会杰.氯化胆碱对受旱地黄叶片渗透调节物质与光合的影响[J].河南农业科学,2007(1):84-86.

[7] Stephen M,Poling W H.Chemical induction of β-carotene biosynthesis[J].Phytochemistry,1977,16:551-555.

[8] Stephen M,Poling W H.Synthetic bioregulators of poly-cis-carotenoid biosynthesis[J].Phytoehemistry,1982,21 (3):601 -604.

[9] Brown R H.Influence of sueeinic acid 2,2-dimethylhtdrazlde on yield and morphological eharaeteristle of starve peanut (Arachis hypogaea L.)[J].Crop Science,1973,13(5):507 -510.

[10] Gepstein S,Nooden I D,Leopold A C.Senescence and sging in plant[M].New York:Academic press,1988:85-109.

[11] 张宪政.作物生理研究法[M].北京:农业出版社,1993.

[12] 刘祖祺.植物抗性生理学[M].北京:中国农业出版社,1994.

[13] 王晶英.植物生理生化实验技术与原理[M].哈尔滨:东北林业大学出版社,2000.

[14] 张志良主编.植物生理学实验指导[M].北京:高等教育出版社,2001.

[15] Vernon A W.Effect of pod removal on leaf senescence in soybeans[J].Plant Physiol,1982,70:1544-1548.

[16] Azada K.The water-water cycle in choroplasts:scavenging of active oxygens and dissipation of excess photons[J].Annu Rev Plant Physiol Plant Mol Biol,1999,50:601 -639.

[17] Foyer C H,Nocter G.Oxygen processing in photosynthesis:regulation and signaling[J].New Phytol,2000,146:359-388.

[18] Palma J M,Jim nez A,Sandalio L M,et al.Anfioxidafive en-zymes from chloroplasts,mitochondria,and peroxisomes dur-ing leaf senescence of nodulated pea plants[J].Exp Bot,2006,57:1747-1758.

[19] O'Kane D,Gill V,Boyd P,et al.Chilling,oxidative stress and antioxidant response in Arabidopsis thaliana callus[J].Planta,1996,198:371-377.

[20] Rivero R M,Ruiz J M,Garefa P C,et al.Response of oxida-tive metabolism in watermelon plants subjected to cold stress[J].Funct Plant Biol,2002,29:643-648.

[21] Lyons J M.Chilling injury in plants[J].Annu Rev Plant Physiol,1973,24:445-466.

[22] Agati G,Mazzinghi P,Lipueci Di,et al.The F685/F730 chlorophyll fluorescence ratio as indicator of drilling stress in plants[J].J Plant Physiol,1996,148:384-390.

[23] Kudah H,Sonoike K.Ireversible damage to photosystem I by chilling in the light:cause of the degradation of chlorophyll after returning to normal growth temperapture[J].Planta,2002,215:541-548.

[24] Lu C M,Lu Q G,Zhang J H,et al.Characterization of pho-tesynthetic pigment compositlon,photesystem Ⅱ photochem-istry and thermal energy dissipation during leaf senescence of wheat plants grown in the field[J].J.Exp Bot,2001,52:1805-1810.

[25] 王月福,于振文,李尚霞.氮素营养水平对小麦旗叶衰老过程中蛋白质和核酸代谢的影响[J].植物营养与肥料学报,2003,9(2):178-183.

[26] Hisao T C.Physiological effects of plant in response to water stress[J].Ann Rev Plant Physiol,1973,24:519-570.

Please choose a citation manager

Content to export