Identification and Mapping of Wheat Leaf Rust Resistance Gene in 06Dn23/Henong 5290 RIL Population

doi:10.7668/hbnxb.20196538

Abstract

Abstract:

Wheat leaf rust is a significant disease affecting wheat production in China.The Hebei wheat cultivar Henong 5290 exhibits good resistance to wheat leaf rust.190 recombinant inbred lines(RILs)derived from a cross between 06Dn23 and Henong 5290 through consecutive selfing were used as experimental materials for mapping leaf rust resistance genes.Seedling-stage resistance identification was first conducted on this RIL population using artificial inoculation.Combined with molecular marker detection and wheat 55K SNP array scanning,linkage analysis was performed to mine seedling-stage leaf rust resistance genes.Subsequently,multi-environment investigations of leaf rust resistance at the adult plant stage were carried out.By integrating field phenotypic data and genotypic data,quantitative trait locus(QTL)mapping for leaf rust resistance at both seedling and adult plant stages was completed in this RIL population.The results showed that the known seedling resistance genes Lr1 and Lr13,and one novel gene,temporarily designated LrHN52,were identified in the population.QTL mapping at the adult plant stage revealed four leaf rust resistance QTLs located on wheat chromosomes 1B,5A,and 7D,temporarily named QLr.hbau-1B,QLr.hbau-5A,QLr.hbau-7D.1,and QLr.hbau-7D.2.Among them,QLr.hbau-1B was stably identified across all environments and was close to the previously reported QLr-WorldAdult-1BS.2 locus,suggesting it may be the same QTL.QLr.hbau-5A was stably expressed in all environments and was proposed to be a novel leaf rust resistance QTL.QLr.hbau-7D.1 was detected in only a single environment and may represent a novel locus.Through marker detection and analysis of the physical positions of linked SNP markers,QLr.hbau-7D.2 was confirmed to be the known leaf rust resistance gene Lr34.

Key words: Wheat, Leaf rust resistance gene, Molecular marker detection, QTL mapping

CLC Number:

WANG Yangang, WEI Yue, DONG Rui, LI Jiawang, ZHANG Zhenxin, LI Yaning, ZHANG Peipei, ZHANG Yelun, LI Zaifeng. Identification and Mapping of Wheat Leaf Rust Resistance Gene in 06Dn23/Henong 5290 RIL Population[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(5): 208-217. doi: 10.7668/hbnxb.20196538.

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Figures/Tables 11

References 40

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基因/品种 Gene/Cultivar	叶锈菌小种 Puccinia triticina races
基因/品种 Gene/Cultivar	PKST	FHGQ	FHJQ	TKSS	PKJS	PHDS	PKTT	THNG	FJJQ	FHGS	FHSS	SHJT
Lr1	4	1	1	4	3	4	3+	4	1	2	1	4
Lr2a	1	1+	2+	3	1	1	2	4	1	2	1+	4
Lr2c	3	3+	4	4	3	3+	4	4	4	4	3+	4
Lr3	4	4	4	4	4	4	4	4	4	4	4	2
Lr9	0	0	0	0	0	0	0	0	0	0	0	0
Lr16	3+	4	4	4	4	4	4	4	4	4	4	3
Lr24	4	0	0	4	4	;	3+	;	4	;	;	;
Lr26	4	4	4	4	4	4	4	4	;	3+	3	4
Lr3ka	4	2	2	3	2	1	3	3-	2	1	3	2
Lr11	4	3+	4	3	4	2	4	2	3	3+	3+	4
Lr17	4	2	4	3	3+	3+	3+	3+	3	2	3	4
Lr30	2	1	1	2	1	1	3+	1	1	1	1	2
LrB	4	4	4	3+	4	2+	4	2	4	3+	4	4
Lr10	4	4	4	4	4	3	3+	3+	3+	3+	3+	4
Lr14a	4	2	2	3+	2+	3+	3+	x	2	3+	3	3+
Lr18	3+	1	1	2	2	1	3+	2	2	2	1	3
Lr2b	1+	2+	2	3+	3+	1	2	3	2	3	2	4
Lr3bg	4	3+	4	3	4	4	3+	3+	3	3	4	2
Lr13	2	2	2	2+	2+	4	3+	2+	3	3	2+	2
Lr14b	4	4	4	4	4	4	1	3+	;	3	4	3+
Lr15	;	;	;	4	2	4	;	3+	;	;	3	1
Lr19	;	0	0	0	0	0	0	;	0	0	0	0
Lr20	2;	;	3	1	3	1	4	4	3	4	3+	3+
Lr21	3+	3	3	2	4	3	3+	3	3	3	1	1
Lr23	4	3	3+	3	3+	3	3	2	3	3+	3+	3
Lr28	0	0	0	0	0	0	0	0	0	0	0	0
Lr29	;	;	;	0	;	1	1	1	1	2	1	2+
Lr33	4	3+	4	4	3+	3	3+	3+	3	3	3+	3+
Lr36	2	1	1	2	2	3+	2	2	2	1+	2	2
Lr39	4	3+	4	3+	4	3+	3+	3	3+	3+	3+	4
Lr42	1	2	1	;	1	3+	2	2	1	1	1	1
Lr44	1	3+	4	2	1	1	1	3	3	3	3	3+
Lr45	1	3+	1	4	1	1	3	3	2	3	3	3+
Lr47	4	3	3	4	;	3	3+	3+	3	3	4	3+
Lr51	;	;	;	;	0	;	;	;	;	;	;	;
Lr53	0	0	0	0	0	0	0	0	0	0	0	0
06Dn23	4	;	1	4	4	4	4	3+	;	2	1	4
河农5290 Henong 5290	2	1	2	2	2	3	2	2	1	1	1	2

基因/品种 Gene/Cultivar	叶锈菌小种 Puccinia triticina races
基因/品种 Gene/Cultivar	PKST	FHGQ	FHJQ	TKSS	PKJS	PHDS	PKTT	THNG	FJJQ	FHGS	FHSS	SHJT
Lr1	4	1	1	4	3	4	3+	4	1	2	1	4
Lr2a	1	1+	2+	3	1	1	2	4	1	2	1+	4
Lr2c	3	3+	4	4	3	3+	4	4	4	4	3+	4
Lr3	4	4	4	4	4	4	4	4	4	4	4	2
Lr9	0	0	0	0	0	0	0	0	0	0	0	0
Lr16	3+	4	4	4	4	4	4	4	4	4	4	3
Lr24	4	0	0	4	4	;	3+	;	4	;	;	;
Lr26	4	4	4	4	4	4	4	4	;	3+	3	4
Lr3ka	4	2	2	3	2	1	3	3-	2	1	3	2
Lr11	4	3+	4	3	4	2	4	2	3	3+	3+	4
Lr17	4	2	4	3	3+	3+	3+	3+	3	2	3	4
Lr30	2	1	1	2	1	1	3+	1	1	1	1	2
LrB	4	4	4	3+	4	2+	4	2	4	3+	4	4
Lr10	4	4	4	4	4	3	3+	3+	3+	3+	3+	4
Lr14a	4	2	2	3+	2+	3+	3+	x	2	3+	3	3+
Lr18	3+	1	1	2	2	1	3+	2	2	2	1	3
Lr2b	1+	2+	2	3+	3+	1	2	3	2	3	2	4
Lr3bg	4	3+	4	3	4	4	3+	3+	3	3	4	2
Lr13	2	2	2	2+	2+	4	3+	2+	3	3	2+	2
Lr14b	4	4	4	4	4	4	1	3+	;	3	4	3+
Lr15	;	;	;	4	2	4	;	3+	;	;	3	1
Lr19	;	0	0	0	0	0	0	;	0	0	0	0
Lr20	2;	;	3	1	3	1	4	4	3	4	3+	3+
Lr21	3+	3	3	2	4	3	3+	3	3	3	1	1
Lr23	4	3	3+	3	3+	3	3	2	3	3+	3+	3
Lr28	0	0	0	0	0	0	0	0	0	0	0	0
Lr29	;	;	;	0	;	1	1	1	1	2	1	2+
Lr33	4	3+	4	4	3+	3	3+	3+	3	3	3+	3+
Lr36	2	1	1	2	2	3+	2	2	2	1+	2	2
Lr39	4	3+	4	3+	4	3+	3+	3	3+	3+	3+	4
Lr42	1	2	1	;	1	3+	2	2	1	1	1	1
Lr44	1	3+	4	2	1	1	1	3	3	3	3	3+
Lr45	1	3+	1	4	1	1	3	3	2	3	3	3+
Lr47	4	3	3	4	;	3	3+	3+	3	3	4	3+
Lr51	;	;	;	;	0	;	;	;	;	;	;	;
Lr53	0	0	0	0	0	0	0	0	0	0	0	0
06Dn23	4	;	1	4	4	4	4	3+	;	2	1	4
河农5290 Henong 5290	2	1	2	2	2	3	2	2	1	1	1	2

侵染型 Infection types	症状 Symptoms
0	无可见的侵染点或孢子堆
;	有褪绿斑或坏死,但是无孢子堆
1	有坏死或者有褪绿斑但孢子堆很小
2	有坏死或者有褪绿斑且孢子堆中等偏小
X	不同大小的孢子堆随机分布
3	叶片有或无褪绿斑,孢子堆中等大小
4	无褪绿斑或者坏死,孢子堆大

侵染型 Infection types	症状 Symptoms
0	无可见的侵染点或孢子堆
;	有褪绿斑或坏死,但是无孢子堆
1	有坏死或者有褪绿斑但孢子堆很小
2	有坏死或者有褪绿斑且孢子堆中等偏小
X	不同大小的孢子堆随机分布
3	叶片有或无褪绿斑,孢子堆中等大小
4	无褪绿斑或者坏死,孢子堆大

染色体 Chromosome	标记数量 Number of Markers	遗传长度/cM Genetic distance	标记密度/cM Marker density	染色体 Chromosome	标记数量 Number of Markers	遗传长度/cM Genetic distance	标记密度/cM Marker density
1A	44	106.20	2.41	4D	54	112.32	2.08
1B	77	146.80	1.91	5A	157	151.40	0.96
1D	42	57.30	1.36	5B	61	109.91	1.80
2A	72	106.92	1.49	5D	81	168.20	2.08
2B	88	133.10	1.51	6A	47	40.16	0.85
2D	55	179.37	3.26	6B	74	85.94	1.16
3A	85	233.06	2.74	6D	84	118.66	1.41
3B	62	131.86	2.13	7A	73	139.90	1.92
3D	38	120.53	3.17	7B	102	135.35	1.33
4A	60	64.67	1.08	7D	68	160.90	2.37
4B	38	88.01	2.32

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