Effects of Carbon and Nitrogen Input on Organic Phosphorus and Extracellular Enzyme Activities in Four Different Land Use Soils

doi:10.7668/hbnxb.20193615

Abstract

Abstract:

Soil phosphorus(P)cycle are varied in different land use soils.The transformation of soil organic P by hydrolyzing by enzymes is an important process in soil P cycling.The exogenous carbon(C)and nitrogen(N)application can influence on the biological process of soil P,which could play a key role in improving soil P bioavailability.In order to study the effects of exogenous C and N on soil organic P and extracellular enzyme activities in different land use soils,four soils from greenhouse vegetable field,grain field,grassland and forest were collected to conduct the incubation experiment.For each soil,four treatments were designed,including control(CK),glucose(G,500 mg/kg soil),potassium nitrate(N,300 mg/kg soil),glucose and potassium nitrate(GN,500 mg/kg glucose soil and 300 mg/kg potassium nitrate soil).The results showed that the soil organic P in greenhouse vegetable field and forest was increased after adding carbon source,increased by 89.7%,40.6%,respectively,compared to control treatment;with the addition of nitrogen sources,the available P in greenhouse vegetable soil increased by 14.2%,while that in forest soil decreased by 14.0%;compared to control treatment,the activity of alkaline phosphatase in grassland and forest soil decreased by 35.9%,25.5% with the input of carbon source,while that in greenhourse vegetable field and grain field soil had no significant effect;compared to control treatment, the β-glucosidase activity of four soils were increased by adding C and N, and the difference was significant in greenhouse vegetable field and grassland soil; based on the addition of carbon source,N addition reduced the cumulative release of CO₂ in grassland and forest soils by 19.7%,16.5%,respectively,but had no significant effect on that of greenhouse vegetable field and grain field soil.Therefore,in greenhouse vegetable field and grain field soil,the soil extracellular enzyme activity increased significantly after the application of exogenous carbon and nitrogen,and the mineralization of organic phosphorus into inorganic phosphorus was more.The content of soil organic carbon was the main factor affecting the mineralization of organic phosphorus in soil;in grassland and forest soil,the application of exogenous carbon and nitrogen reduced the activity of alkaline phosphatase and slowed down the mineralization process of organic phosphorus,so the activity of phosphatase was the main factor affecting the mineralization of organic phosphorus in the soil.

Key words: Soil phosphorus, Phosphatase, Soil culture, β-glucosidase, Soil organic carbon, Carbon and nitrogen input

ZHAO Yuanyuan, JIN Jiawen, CHEN Shuo, ZHANG Shuai, JIN Yuwei, JIANG Jiayi, HAO Xiangrui, ZHANG Delong, CHEN Qing. Effects of Carbon and Nitrogen Input on Organic Phosphorus and Extracellular Enzyme Activities in Four Different Land Use Soils[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(3): 139-147. doi: 10.7668/hbnxb.20193615.

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References 41

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土壤 Soil	pH	电导率/ (μS/cm) Electric conductivity	CaCO₃/ (g/kg)	速效磷/ (g/kg) Available phosphorus	总磷/ (g/kg) Total phosphorus	有机碳/ (g/kg) Organic carbon	总氮/ (g/kg) Total nitrogen	OC/TN	OC/TP
菜田Greenhouse vegetable field	7.36	265	25.60	175.00	1.23	10.40	0.93	19.24	14.63
粮田Grain field	7.73	177	29.90	6.54	0.58	8.03	0.78	17.72	23.70
草地Grassland	6.12	318	1.00	8.96	0.32	17.70	1.97	15.50	96.75
森林Forest	6.31	253	2.54	20.40	1.20	48.70	2.99	28.14	70.27

土壤 Soil	pH	电导率/ (μS/cm) Electric conductivity	CaCO₃/ (g/kg)	速效磷/ (g/kg) Available phosphorus	总磷/ (g/kg) Total phosphorus	有机碳/ (g/kg) Organic carbon	总氮/ (g/kg) Total nitrogen	OC/TN	OC/TP
菜田Greenhouse vegetable field	7.36	265	25.60	175.00	1.23	10.40	0.93	19.24	14.63
粮田Grain field	7.73	177	29.90	6.54	0.58	8.03	0.78	17.72	23.70
草地Grassland	6.12	318	1.00	8.96	0.32	17.70	1.97	15.50	96.75
森林Forest	6.31	253	2.54	20.40	1.20	48.70	2.99	28.14	70.27

处理 Treatment		pH值	有机碳/ (g/kg) Organic carbon	全氮/ (g/kg) Total nitrogen	总磷/ (g/kg) Total phosphorus	OC/TN	OC/TP
菜田	CK	6.85±0.03a	11.30±2.28a	0.88±0.01b	1.24±0.01a	12.80±2.58a	9.11±1.85a
Greenhouse	G	6.87±0.06a	9.65±0.77a	0.86±0.03b	1.32±0.00a	11.20±0.57ab	7.33±0.57a
vegetable field	N	6.76±0.02b	9.84±0.59a	0.98±0.03a	1.25±0.02a	10.00±0.29b	7.84±0.41a
	GN	6.72±0.02b	10.90±0.94a	1.08±0.10a	1.47±0.30a	10.10±0.26b	7.52±1.05a
粮田	CK	7.22±0.04a	8.65±0.38a	0.71±0.02b	0.67±0.01a	12.20±0.65a	13.00±0.73a
Grain field	G	7.30±0.02a	8.79±0.25a	0.73±0.05b	0.65±0.05a	12.00±0.84a	13.60±1.36a
	N	7.10±0.02b	8.58±0.27a	0.83±0.08ab	0.57±0.09a	10.40±0.76b	18.50±2.33a
	GN	7.12±0.08b	8.66±0.17a	0.94±0.08a	0.64±0.02a	9.28±0.73b	13.50±0.22a
草地	CK	5.40±0.12b	20.70±0.80a	2.21±0.17b	0.42±0.02a	9.38±0.83a	49.50±2.35a
Grassland	G	5.49±0.09b	20.90±1.34a	2.34±0.11ab	0.43±0.03a	8.95±0.69ab	48.40±2.39a
	N	5.71±0.01a	21.70±1.56a	2.58±0.12a	0.42±0.03a	8.42±0.50ab	51.50±2.51a
	GN	5.83±0.06a	19.90±1.65a	2.63±0.26a	0.43±0.03a	7.62±0.92b	47.00±4.74a
森林	CK	5.68±0.03a	42.40±1.69a	3.27±0.23b	1.33±0.02a	13.00±1.01a	32.00±1.65a
Forest	G	5.69±0.04a	44.00±2.16a	3.37±0.12b	1.32±0.03a	13.00±0.38a	33.40±1.85a
	N	5.68±0.02a	45.10±1.61a	3.72±0.23a	1.32±0.05a	12.20±0.81a	34.30±1.31a
	GN	5.73±0.07a	46.70±3.43a	3.89±0.11a	1.32±0.00a	12.00±1.08a	35.30±2.48a

处理 Treatment		pH值	有机碳/ (g/kg) Organic carbon	全氮/ (g/kg) Total nitrogen	总磷/ (g/kg) Total phosphorus	OC/TN	OC/TP
菜田	CK	6.85±0.03a	11.30±2.28a	0.88±0.01b	1.24±0.01a	12.80±2.58a	9.11±1.85a
Greenhouse	G	6.87±0.06a	9.65±0.77a	0.86±0.03b	1.32±0.00a	11.20±0.57ab	7.33±0.57a
vegetable field	N	6.76±0.02b	9.84±0.59a	0.98±0.03a	1.25±0.02a	10.00±0.29b	7.84±0.41a
	GN	6.72±0.02b	10.90±0.94a	1.08±0.10a	1.47±0.30a	10.10±0.26b	7.52±1.05a
粮田	CK	7.22±0.04a	8.65±0.38a	0.71±0.02b	0.67±0.01a	12.20±0.65a	13.00±0.73a
Grain field	G	7.30±0.02a	8.79±0.25a	0.73±0.05b	0.65±0.05a	12.00±0.84a	13.60±1.36a
	N	7.10±0.02b	8.58±0.27a	0.83±0.08ab	0.57±0.09a	10.40±0.76b	18.50±2.33a
	GN	7.12±0.08b	8.66±0.17a	0.94±0.08a	0.64±0.02a	9.28±0.73b	13.50±0.22a
草地	CK	5.40±0.12b	20.70±0.80a	2.21±0.17b	0.42±0.02a	9.38±0.83a	49.50±2.35a
Grassland	G	5.49±0.09b	20.90±1.34a	2.34±0.11ab	0.43±0.03a	8.95±0.69ab	48.40±2.39a
	N	5.71±0.01a	21.70±1.56a	2.58±0.12a	0.42±0.03a	8.42±0.50ab	51.50±2.51a
	GN	5.83±0.06a	19.90±1.65a	2.63±0.26a	0.43±0.03a	7.62±0.92b	47.00±4.74a
森林	CK	5.68±0.03a	42.40±1.69a	3.27±0.23b	1.33±0.02a	13.00±1.01a	32.00±1.65a
Forest	G	5.69±0.04a	44.00±2.16a	3.37±0.12b	1.32±0.03a	13.00±0.38a	33.40±1.85a
	N	5.68±0.02a	45.10±1.61a	3.72±0.23a	1.32±0.05a	12.20±0.81a	34.30±1.31a
	GN	5.73±0.07a	46.70±3.43a	3.89±0.11a	1.32±0.00a	12.00±1.08a	35.30±2.48a

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