Abstract: To investigate the physiological function of a cold-and drought-inducible gene AmZFP1 (Zinc finger protein 1) from Ammopiptanthu smongolicus with strong stress tolerance,the expression analysis of AmZFP1 was performed by using the semi-quantitative RT-PCR method.The results showed that in the seedlings cultivated in normal growth conditions,a relatively high expression level of AmZFP1 was detected.After exposing the seedlings to low temperature or dehydration treatment between 2 to 48 hours,the expression level of AmZFP1 increased markedly,and this up-regulation was more rapid and slightly higher during the dehydration treatment.In the young leaves,flower buds and immature pods of the A. mongolicus plants grown in the wild,the expression levels of AmZFP1 were quite high,whereas in the twigs and roots,the transcripts of AmZFP1 were not detected.In young leaves of the wild grown A. mongolicus plants,the expression level of AmZFP1 was obviously high in the cold winter but was low or quite weak in the warm or hot seasons,including spring,summer and autumn.Moreover,the coding region cDNA of AmZFP1 was cloned by RT-PCR,whose predicted polypeptide contains two typical C2H2 type zinc finger domains and one nuclear localization signal.The cDNA fragment was also successfully inserted into the plant expression vector p3300-35ST.This work laid a foundation for further functional analysis of AmZFP1.

Key words: Ammopiptanthus mongloicus, ZFPs, Expression analysis, Gene cloning

CLC Number: 

• Q78