Abstract: In order to study the function and molecular mechanism of CHS.A CHS gene was cloned from the mango fruit cDNA and DNA,by homology cloning method.The open reading frame was 1 194 bp,and encoding 397 amino acids,its isoelectric point was 6.03,the molecular weight was 43.29 kDa.The 1 315 bp fragment was amplified from the genome,the result showed that the gene contained a intron by analysis.The expression of CHS gene was analysis in young leaves and mature leaves,it found that the gene was expressed in different period leaves,and higher in the red young leaves.It was inferred that the gene might be involved in the flavonoids synthesis of leaves.It was found that the gene encoding for the protein had close relationship with Paphiopedilum concolor, Bletilla striata,through phylogenetic analysis,considered as a group with Bletilla striata, Equisetum arvense and, Brassica oleracea.

Key words: Mango, CHS, Gene cloning, Expression analysis

CLC Number: 

• Q78

• S667.7