Isolation and Regulation Analysis of GmNAC011 Gene to Isoflavone Synthase Gene GmIFS2

doi:10.7668/hbnxb.2015.05.004

Abstract

Abstract: Based on the previous results of transcriptome sequencing,the expression of GmNAC011 was different in soybean cultivars that differed in isoflavonoid accumulation,thus we selected this gene for further analysis.Primers were designed according to the soybean genome sequences.The whole opening reading frame of GmNAC011 was isolated from soybean roots,encoding 268 amino acids with a calculated molecular mass of 31 kDa and a theoretical pI of 8.3. Phylogenetic analysis showed that GmNAC011 was close to GmNAC2,belonging to ATAF1-like NAC transcription factor subgroup.We investigated the expression pattern of GmNAC011 and GmIFS2 in various tissues(root,stem,leaf,flower and pod),the expression model of GmNAC011 was similar with GmIFS2. The transcript levels of GmNAC011 and GmIFS2 were found to be higher in roots,stems,leaves and pods than flowers.And we found that GmNAC011 could binding to the motif "CGTG" in the promoter of GmIFS2 by yeast one-hybrid.All results showed that GmNAC011 participate in regulating the expression of GmIFS2,subsequently the synthesis of isoflavonoid.

Key words: Soybean, Transcription factor, GmNAC011, GmIFS2

CLC Number:

S565.01
Q78

LIU Xiao-qing, CHEN Hua-tao, ZHANG Hong-mei, YUAN Xing-xing, CUI Xiao-yan, GU He-ping, CHEN Xin. Isolation and Regulation Analysis of GmNAC011 Gene to Isoflavone Synthase Gene GmIFS2[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 25-29. doi: 10.7668/hbnxb.2015.05.004.

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