Cloning and Expression Analysis of a bZIP Transcription Factor Gene, RjbZIP,from Racomitrium japonicum

doi:10.7668/hbnxb.2015.04.003

Abstract

Abstract: In order to study biological function of bZIP transcription factor in bryophytes,a full length cDNA sequence of bZIP transcription factor was cloned from Racomitrium japonicum using RT-PCR,named RjbZIP.The full length of RjbZIP cDNA sequence was 1 477 bp,containing a 1 422 bp open reading frame (ORF),and encoding a protein of 473 amino acids,molecular weight was 5.114 kDa and theoretical pI was 6.97.The predicted RjbZIP protein belonged to unstable protein,and located in nuclues without transmembrane structure and signal peptide.The protein contained a typical structure of bZIP domain,including one leucine zipper motif,one basic domain and one glutamine rich.domain.The quantitative RT-PCR results showed that RjbZIP gene could express in the proceed of quick dehydration,slow dehydration and rehydration,and its transcriptional responses subject to rehydration was the most sensitive.Therefore,the RjbZIP gene was speculated to participate the stress reaction of R.japonicum,and the results laid the foundations for the further study on its function.

Key words: Racomitrium japonicum, bZIP transcription factor, RjbZIP gene, Gene cloning, Expression analysis

CLC Number:

SHA Wei, ZHANG Mei-juan, LIU Bo, XU Hong-hong. Cloning and Expression Analysis of a bZIP Transcription Factor Gene, RjbZIP,from Racomitrium japonicum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 13-20. doi: 10.7668/hbnxb.2015.04.003.

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