Genetic Analysis and Gene Mapping of a Leaf Mutant in Maize

doi:10.7668/hbnxb.2011.03.002

Abstract

Abstract: Natural and artificial mutants are as valuable resources for functional genomics and molecular breeding.A leaf-color mutant was identified in progency of a maize inbred line 4H8.Under natural conditions,it was virescent and growed slowly at seedings,then wilted to die gradually.A set of segregating populations derived from two inbred lines,the parents 4H8 and 40-6,were used to study the genetic mechanism of virescent leaf in maize.The result demonstrated that the trait of virescent leaf in the inbred line 4H8 was governed by a recessive gene(vl1),which was mapped on the long arm of chromosome 1 by SSR analysis,the vl1 was flanked by two SSR markers umc1323 and umc1709,with the genetic distances 2.1 cM and 10.3 cM,respectively.The results will be useful for cloning and functional analysis of it.

Key words: Maize, A leaf mutant, Genetic analysis, Gene mapping, Molecular marker

CLC Number:

CHENG Hong-liang, CHEN Jia-fa, DING Jun-qiang, WU Jian-yu. Genetic Analysis and Gene Mapping of a Leaf Mutant in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 7-10. doi: 10.7668/hbnxb.2011.03.002.

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References

[1] 郭建秋,雷全奎,杨小兰,等.植物突变体库的构建及突变体检测研究进展[J].河南农业科学,2010(6):150-155.

[2] 王凤华,李光远.T-DNA插入突变及其研究进展[J].河南农业科学,2007(6):12-14.

[3] 黄晓群,赵海新,董春林,等.水稻叶绿素合成缺陷突变体及其生物学研究进展[J].西北植物学报,2005,25(8):1685-1691.

[4] Lonosky P M,Zhang X,Honavar V G,et al.A proteomic analysis of maize chloroplast biogenesis[J].Plant Physiol,2004,134(2):560-574.

[5] Alberte R S,Hesketh J D,Hofstra G,et al.Compositon and activity of the photosynthetic apparatus intemperature sensitive mutants of higher plants[J].Proc Natl Acad Sci USA,1974,71(6):2414-2418.

[6] 刘冬梅,丁锦平,李成伟,等.棉花突变体的获得及其应用研究进展[J].河南农业科学,2009(11):11-15.

[7] Bae C,Abe T,Matsuyama T,et al.Regulation of chloroplast gene expression is affected in ali,a novel tobacco albino mutant[J].Annals of Botany,2001,88:545-553.

[8] Stockinger E J,Walling L L.A chlorophyll a/b-binding protein gene from soybean (Glycine max[L.] Merr.)[J].Plant Physiol,1994,104(4):1475-1476.

[9] Maneesha R A,Fei Y,Aigen F,et al.Arabidopsis variegation mutants:new insights into chloroplast biogenesis[J].Journal of Experimental Botany,2006,57 (9):1871-1881.

[10] Wu Z,Zhang X,He B,et al.A Chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll Biosynthesis[J].Plant Physiology,2007,145(1):29-40.

[11] Jung K H,HurJ H,Ryu C H,et al.Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system[J].Plant Cell Physiol,2003,44(5):463-472.

[12] 李子芳,宋东颖,张红影,等.水稻T-DNA插入群体的建立及突变体筛选[J].华北农学报,2009,24(2):51-54.

[13] 陈佳颖,赵剑,刘晓,等.一个新水稻温敏感叶色突变体的遗传分析及其基因分子定位[J].植物学报,2010,45(4):419-425.

[14] 马志虎,颜素芳,罗秀龙,等.辣椒黄绿苗突变体对良种繁育及纯度鉴定作用[J].北方园艺,2001,3:13-14.

[15] Wu D X,Sbu Q Y,Xia Y W.In vitro mutagenesis induced novel thermo/photoperiod-sensitive genic male sterile indica rice with green-revertible xanthan leaf color marker[J].Euphytica,2002,123:195-202.

[16] 邢才,王贵学,黄俊丽,等.植物叶绿素突变体及其分子机理的研究进展[J].生物技术通报,2008,5:10-12.

[17] 陈甲法.一个玉米叶色突变体的遗传研究[D].郑州:河南农业大学,2009.

[18] Sanguinetti C J,Dias Neto E,Simpson A J.Rapid silver staining and recovery of PCR products separated on polyacrylamide gel[J].Biotechniques,1994,17 (5):915-919.

[19] Stam P.Construction of integrated genetic linkage maps by means of a new computer package:JoinMap[J].Plant Journal,1993,3:739-744.

[20] 刘仁虎,孟金陵.MapDraw,在Excel中绘制遗传连锁图的宏[J].遗传,2003,25(3):317-321.

[21] Michlmore R W,Papan I,Kesseli R V.Identification of markers linked to disease-resistance genes by bulked segregant analysis:A rapid method to detect markers in specific genomic regions by using segregating populations[J].Proc Natl Acad Sci USA,1991,88:9828-9832.

[22] 王益军,苗楠,施亚婷,等.一份玉米显性矮杆突变体的遗传分析[J].华北农学报,2010,25(5):93-96.

[23] 陈岭,崔绍平,徐有,等.玉米株型性状的遗传分析[J].华北农学报,1994,9(51):14-18.

[24] Maize Genetics and Genomics Database[DB/OL].http://www.maizegdb.org/stock.php.

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