Effect of Low Temperature on Protective Enzyme Activities and Hydrogen Peroxide Production in Spikelet of Rice at Flowering Stage

doi:10.7668/hbnxb.2010.S2.015

Abstract

Abstract: "The experiments were carried out in growth chambers to investigate the effects of low temperature stress during flowering period on protective enzyme activities and hydrogen peroxide production of spikelet using two rice cultivars including low-temperature tolerant cultivar 996 and low-temperature sensitive cultivar 4628.Seven day/night two treatments of optimal temperature(24℃(06:00-8:00;19:00-23:00)/26℃(08:00-10:00;16:00-19:00)/30℃(10:00-16:00)/22℃(23:00-06:00)) and low temperature(19℃(06:00-8:00;19:00-23:00)/21℃(08:00-10:00;16:00-19:00)/23℃(10:00-16:00)/17℃(23:00-06:00)) after heading were established.The results showed that superoxide dismutase(SOD),catalase(CAT),peroxidase(POX) and ascorbic acid-peroxidase(AsA-POX) activcities in spikelet of rice increased obviously at early stage of low temperature stress,then decreased rapidly.The contents of malondialdehyde(MDA) and hydrogen peroxide(H2O2) were increased remarkably with the overtime of low temperature.The increase of MDA content and H2O2 content in the cultivar 4628 was greater than that in the cultivar 996,which may relation with protective enzyme activities in the cultivar 996 were higher than that in the cultivar 4628 under low temperature stress.The above results indicated that the reactive oxygen metabolism of the cultivar 996 was more balanced than that in the cultivar 4628 under low temperature stress. "

Key words: R ice(Oryza sa tiva ), Low temperature stress, Spike let, Protective enzyme, Hydrogen peroxide

CLC Number:

S511.01

DENG Hua-bing, WANG Tian-shun, XIAO Ying-hui, TANG Wen-bang, PAN Yi, CHEN Li-yun. Effect of Low Temperature on Protective Enzyme Activities and Hydrogen Peroxide Production in Spikelet of Rice at Flowering Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S2): 62-67. doi: 10.7668/hbnxb.2010.S2.015.

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References

[1] 孔佳良,余冬林.低温冷害对湖南晚稻危害特点及调控技术[J].湖南农业科学,2009(7):67-69.
[2] 叶昌荣,戴陆园,王建军,等.低温冷害影响水稻结实率的要因分析[J].西南农业大学学报,2000,22(4):307-309.
[3] 叶昌荣,戴陆园,廖新华,等.低温诱导下水稻花药和不育花粉数的变化及其与耐泠性的关系[J].西南农业学报,1996,9(3):1-6.
[4] 施大伟,张成军,陈国祥,等.低温对高产杂交稻抽穗期剑叶光合色素含量和抗氧化酶活性的影响[J].生态与农村环境学报,2006,22(2):40-44.
[5] 王萍,张成军,陈国祥,等.低温对水稻剑叶膜脂过氧化和脂肪酸组分的影响[J].作物学报,2006,32(4):568-572.
[6] 王萍,张成军,陈国样,等.低温对水稻幼苗类囊体膜脂肪酸组分和膜脂过氧化的影响[J].中国水稻科学,2006,20(4):401-405.
[7] 王静,孙磊,张成军,等.杂交稻幼苗期对低温胁迫的生理反应[J].作物学报,2006,32(7):1049-1056.
[8] 李霞,戴传超,焦德茂,等.光照条件下低温对水稻籼梗亚种幼苗抗氧化物质含量的影响[J].植物生理与分子生物学学报,2006,32(3):345-353.
[9] 邓化冰,肖应辉,唐文帮,等.两系杂交早稻父本996孕穗期和开花期耐冷性评价[J].杂交水稻,2009,24(6):62-65.
[10] 李合生,孙群,赵世杰,等.植物生理生化试验原理和技术[M].北京:高等教育出版社,2000:167-169.
[11] Scebba F,Sebastiani L.Vitagliano C.Protective enzymes against activated oxygen species in wheat(Triticum aestivum L.)seedling:response to cold accilimation[J].Plant Physiol,1999,155:762 -768.
[12] 张宪政.作物生理研究法[M].北京:农业出版社,1992:201-212,197-198.
[13] Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxides in spinach chloroplasts[J].Plant Cell Physiol,1981,22:867-880.
[14] Health R L,Pakcer L.Photoperoxidation in isolated chloroplasts.I.Kinetics and stoichiometry of factty acid peroxidation[J].Arch Biochem Biophys,1968,125:189-198.
[15] Patterson B D,Macrae E A,Ferguson I B.Estimation of hydrogen peroxidein plant extracts using titanium(Ⅳ)[J].Anal Biochem,1984,134:487-492.
[16] 隗溟,王光明,陈国惠,等.盛花期高温对两系杂交稻两优培九结实率的影响[J].杂交水稻,2002,17(1):51-53.
[17] 肖用森,王正直,郭绍川.低温胁迫对杂交稻及其亲本幼苗内活性氧清除剂的影响[J].杂交水稻,1990,5(5):39-42.
[18] 刘家忠,龚明.植物抗氧化系统研究进展[J].云南师范大学学报,1999,19(6):1-11.
[19] Prasad T K.Mechanism of chilling-induced of dative stress injury and tolerance:change in anitioxidant system,oxidation of proteins and lipids and protease activities[J].Plant,1996,10:1017-1026.
[20] 王静,张成军,陈国祥,等.低温对灌浆期水稻剑叶光合色素和类囊体膜脂肪酸的影响[J].中国水稻科学,2006,20(2):177-182.
[21] 邵继荣,刘永胜,周仕春,等.冷锻炼对提高水稻幼苗抗寒性及其细胞器膜结构稳定性的影响[J].作物学报,1999,25(5):569-574.
[22] 李美茹,刘鸿先,王以柔,等.低温下水稻幼苗叶片细胞膜膜脂过氧化和膜磷脂脱酯化反应[J].广西植物,1998,18(2):173-176.

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