Effect of Arbuscular Mycorrhizal Fungus on Morphology, Growth and Photosynthetic Characteristics in Maize Seedlings under Low Temperature Stress

doi:10.7668/hbnxb.2014.S1.031

Abstract

Abstract: For understanding the effects and effect mechanisms of arbuscular mycorhizal(AM) fungus on low temperature tolerance of maize.The effect of arbuscular mycorhizal(AM) fungus inoculation on morphology,growth and photosynthetic characteristics of maize seedlings was studied in pot culture under low temperature stress.Study showed that under low temperature stress,compared with non AM-inoculated maize plants,AM-inoculated maize plants had larger leaf size and higher aboveground biomass.Low temperature stress decreased the photosynthetic rate(Pn),stomatal conductance(Gs) and transpiration rate(Tr) of maize leaves,but increased the intercellular CO₂ concentration(Ci).The Pn,Gs and Tr of AM-inoculated maize leaves were higher than those of non AM-inoculated plants,and the Ci of AM-inoculated maize leaves was lower than that of non AM-inoculated plants.Under low temperature stress,the primary fluorescence(Fo) was increased,while variable fluorescence(Fv),maximal fluorescence(Fm),maximum photochemical efficiency(Fv/Fm) and potential photochemical efficiency(Fv/Fo) were decreased.Without regard of temperature,compared with AM-inoculated maize plants,AM-inoculated plants had higher Fv,Fm,Fv/Fm and Fv/Fo.The results indicated that low temperature could induce serious damage of growth and photosynthetic physiology of maize seedling;AM inoculation could enhance low temperature tolerance of maize seedling by protective and facilitated effects on maize plants to promote its growth,and protective effect on photosynthetic system to improve its photosynthetic capacity.

Key words: Low temperature stress, Arbuscular mycorrhiza, Chlorophyll fluorescence, Photosynthesis

CLC Number:

S513.01

CHEN Xiao-ying, SONG Feng-bin, ZHU Xian-can, SUN Lu-ying, MA Fu, LIU Sheng-qun. Effect of Arbuscular Mycorrhizal Fungus on Morphology, Growth and Photosynthetic Characteristics in Maize Seedlings under Low Temperature Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 155-161. doi: 10.7668/hbnxb.2014.S1.031.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.S1.031

http://www.hbnxb.net/EN/Y2014/V29/IS1/155

References

[1] 汪黎明,郭庆法,张发军,等.加入WTO后对我国玉米育种的影响及对策分析[J].玉米科学,2003,11(z2):68-69.
[2] Ma Z Q,Wang Q,Wang C Y,et al.The risk division on climate and economic loss of maize chilling damage in Northeast China[J].Geographical Research,2008,27(5):1169-1177.
[3] 马树庆,刘玉英,王琪.玉米低温冷害动态评估和预测方法[J].应用生态学报,2006,17(10):1905-1910.
[4] 张养才,何维勋,李世奎.中国农业气象灾害概论[M].北京:气象出版社,1991:51-52.
[5] 马树庆,袭祝香,王琪.中国东北地区玉米低温冷害风险评估研究[J].自然灾害学报,2003,12(3):137-141.
[6] 梁宇,高玉葆.内生真菌对植物生长发育及抗逆性的影响[J].植物学通报,2000,17(1):52-59.
[7] 李莉,黄群策,秦广雍.海藻糖在提高植物抗逆性方面的研究进展[J].生物学通报,2003,38(6):6-7.
[8] 杨凤萍,梁荣奇,张立全,等.抗逆调节转录因子 CBF1 基因提高多年生黑麦草的抗旱能力[J].华北农学报,2006,21(1):14-18.
[9] Parniske M.Arbuscular mycorrhiza:the mother of plant root endosymbioses[J].Nature Reviews Microbiology,2008,6(10):763-775.
[10] Smith S E,Read D J.Mycorrhizal symbiosis 3rd edn[M].London:Academic Press,2008.
[11] Augé R M.Water relations,drought and vesicular-arbuscular mycorrhizal symbiosis[J].Mycorrhiza,2001,11(1):3-42.
[12] 朱先灿.丛枝菌根真菌对玉米抗逆性的影响[D].长春:中国科学院东北地理与农业生态研究所,2010.
[13] Zhu X C,Song F B,Liu S Q,et al.Effects of arbuscular mycorrhizal fungus on photosynthesis and water status of maize under high temperature stress[J].Plant and Soil,2011,346(1/2):189-199.
[14] 金樑,陈国良,赵银,等.丛枝菌根对盐胁迫的响应及其与宿主植物的互作[J].生态环境,2007,16(1):228-233.
[15] Phillips J M,Hayman D S.Improved procedure of clearing roots and staining parasiti and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection[J].Transactions of the British Mycological Society,1970,55:159-161.
[16] Gavito M E,Olsson P A,Rouhier H,et al.Temperature constraints on the growth and functioning of root organ cultures with arbuscular mycorrhizal fungi[J].The New Phytologist,2005,168(1):179-188.
[17] 陈笑莹,宋凤斌,朱先灿,等.高温胁迫下丛枝菌根真菌对玉米光合特性的影响[J].华北农学报,2013,28(2):108-113.
[18] Zhang F,Hamel C,Kianmehr H,et al.Root-zone temperature and soybean[ Glycine max.(L.) merr.]vesicular-arbuscular mycorrhizae:Development and interactions with the Nitrogen fixing symbiosis[J].Environmental and Experimental Botany,1995,35(3):287-298.
[19] Liu A,Wang B,Hamel C.Arbuscular mycorrhiza colonization and development at suboptimal root zone temperature[J].Mycorrhiza,2004,14(2):93-101.
[20] Charest C,Dalpé Y,Brown A.The effect of vesicular-arbuscular mycorrhizae and chilling on two hybrids of Zea mays L J］.Mycorrhiza,1993,4(2):89-92.
[21] Anderson C P,Sucoff E I,Dixon R K.The influence of low soil temperature on the growth of vesicular-arbuscular mycorrhizal Fraxinus pennsylvanica [J].Canadian Journal of Forest Research,1987,17(8):951-956.
[22] Gavito M E,Schweiger P,Jakobsen I.P uptake by arbuscular mycorrhizal hyphae:effect of soil temperature and atmospheric CO₂ enrichment[J].Global Change Biology,2003,9(1):106-116.
[23] Volkmar K M,Woodbury W.Effects of soil temperatures and depth on colonization and root and shoot growth of barley inoculated with vesicular-arbuscular mycorrhizae indigenous to Canadian prairie soil[J].Canadian Journal of Botany,1989,67(6):1702-1707.
[24] 吴雪霞,陈建林,查丁石.低温胁迫对茄子幼苗叶片光合特性的影响[J].华北农学报,2008,23(5):185-189.
[25] Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33(1):317-345.
[26] Zhu X C,Song F B,Xu H W.Arbuscular mycorrhizae improves low temperature stress in maize via alterations in host water status and photosynthesis[J].Plant and Soil,2010,331(1/2):129-137.
[27] Fracheboud Y,Haldimann P,Leipner J,et al.Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize( Zea mays L.) J］.Journal of Experimental Botany,1999,50(338):1533-1540.
[28] Baker N R.Chlorophyll fluorescence:a probe of photosynthesis in vivo [J].Annual Review of Plant Biology,2008,59:89-113.

Please choose a citation manager

Content to export