Abstract: Fibroblasts derived from Mengshan cattle ear were firstly successfully cultured with a normal tissue culture method, and then subcuhured for passages, stored in liquid nitrogen and survival rate evaluated after thawing. Finally, cultured cells were analyzed by their morphology, growing curve, chromosomes and cell cycle. Results demonstrated that cultured cells were typical fibroblasts with normal morphology and proliferation. There was no significant difference in the S and G₀+G₁ stage rate of 60% -70% and 80% -90% confluence, but were both higher/ lower than 90% -100% confluence. The survival rates after storage for 1 month and 3 month were higher than storage for 12 month, but storage time had no significant effect on normal chromosomes, S and G₀+G₁ stage rates of cultured cells. In conclusion, the culture of Mengshan cattle ear fibroblasts provided an effective method for conservation of rare and endangered species.

Key words: Mengshan cattle, Fibroblasts, Chromosome, Cell cycle

CLC Number: 

• S823