Virus Elimination and Detection Technique in Red General Fuji Apple Cultivar (Malus domestica)

doi:10.7668/hbnxb.2009.S2.020

Abstract

Abstract: In vitro seedlings subeultured 4 -5 generation of midseason Fuji apple variety Red General were eliminated viruses, and then were detected by ELISA and RT-PCR. Results showed that keeping constant heat treatment conditions at 38℃ for 20 days, the virus elimination rate of Red General apple reached 86.4% -100%, and major apple viruses such as ASSVd, ASGV, ASPV, ApMV and ACLSV were eluted well.

Key words: Apple, Virus elimination, ELISA, RT-PCR

CLC Number:

S661.1

SU Jia-ming, DUAN Xiao-na, YU Qiang, XU Wei-hua, JIANG Zhong-wu, DONG Ya-feng, CHEN Xue-sen. Virus Elimination and Detection Technique in Red General Fuji Apple Cultivar (Malus domestica)[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S2.020.

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http://www.hbnxb.net/EN/Y2009/V24/IS2/93

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