ACTA AGRICULTURAE BOREALI-SINICA

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Construction of Plant Expression Vector of Human Aβ Gene and Its Transformation

HEJian-hua, LIWen-li, WEILi-jun, XIAXiu-ying   

  1. School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China
  • Received:2009-02-05 Published:2009-12-31

Abstract: Human amyloid β peptide (Aβ)is one of the key targets in treatment of Alzheimer′s disease.The two tandem fragment of Aβ obtaining by overlap PCR technique was cloned into plant expression vector pBIDST (containing double 35S promoter and TEV enhancer).The 2Aβ gene was introduced into tobacco by Agrobacterium tumefaciens EHA105. Then the kanamycin resistant tobaccos were obtained.The results of PCR and RT -PCR indicated that the 2Aβ gene was integrated into the genome of tobacco and transcribed.

Key words: AmyloidβPeptide, Plantexpressionvector, Transgenictobacco

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Cite this article

HEJian-hua, LIWen-li, WEILi-jun, XIAXiu-ying. Construction of Plant Expression Vector of Human Aβ Gene and Its Transformation[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.006.

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