Construction of Knockout Vector for sIgM λ Light Chain Gene from DT40 Cells

doi:10.7668/hbnxb.2009.03.001

Abstract

Abstract: β-actin promotor was cloned from chicken B lympha DT40 cells and used to replace the SV40 promotor of pCDNA3. 1( + )vector,so as to construct the Neomycin resistance express cassette driven byβ 2actin promotor. Then about 2 kb DNA sequenceson each side of the locusof sIgMλgene were inserted into the MCSof the constructed cassette,act2 ing as the homologous arm. PCR identification,restriction enzyme digestion and sequence analysis confirmed that the tar2 geting replacement vector pCDNA2act2neO₂HR for sIgMλgene was successfully constructed. This laid the foundation for establishing the model cell lack of sIgMλgene,and further investigating the roles of sIgMλlight chain in the infection of IBDV to DT40 cells.

Key words: sIgMλlight chain, &beta, 2actin promoter, Gene knockout, Homologous recombination

CLC Number:

Q782

YoU Lei ming, LUo Jun, WANG Ai ping, ZHANG Gai ping, BU Dan, Guo Yan an, QI Yan hua. Construction of Knockout Vector for sIgM λ Light Chain Gene from DT40 Cells[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 1-6. doi: 10.7668/hbnxb.2009.03.001.

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