Abstract: :This experiment was aimed to determine the influence of xylazole on Ca2 +-ATPase activities in rats′different brain regions in order to clarify its general anesthetic mechanism.84 Wistar rats were randomly divided into conrol group(12 rats), high dose xylazole group (ip.xylazole 100mg/kg)and low dose xylazole group (ip.xylazole 80 mg/kg), and each dose group was divided into three sub-groups, eg anesthesia group, recovery of righting reflex and aroused group. Rats'brain samples of cerebral cortex, hippocampus, cerebellum and brain stem in different period under xylazole anesthesia were separated by differential centrifugation.The Ca2+-ATPase activities of synaptosome in above brain brain regions were determined by using colorimetry methods.The results showed that, xylazole inhibited Ca 2+-ATPase activities of synaptosomal membranes in cerebral cortex, hippocampus, cerebellum and brain stem in a dose-dependent manners.The inhibition on Ca2+-ATPase activities of synaptosome in these brain regions fitted with the behavioral changes in rats after being administrated with xylazole intraperitoneally.The results indicated that Ca2 +-ATPase in synaptosome in cerebral cortex, hippocampus, cerebellum and brain stem might be the targets of general anesthetic action mediated by xylazole.

Key words: Xylazole, Ca2+-ATPase activities, Anesthesia

CLC Number: 

• S81