Abstract: The allantoic fluid was collected from the dead chicken embryo within 48-96 hours after the 11-day-old SPF brambling chicken embryos by type ZH-1. To amplify the sequence of the fusion gene( F- gene) and to determine whether the amplified sequence contains the integrated sequence of open reading frame(ORF),one pair of specific primer was designed and synthesized basedon the published sequence of the F gene and then was used to amplify the F-gene in brambling by RT-PCR. The amplified fragment was taken back and purified, and then was cleaved by both EcoR I and Sal I enzymes. Later,the F gene was transformed into DH5αby PCI-neo vector. The detected results of positive isolates by both PCRanalysis and enzyme cleaving response with EcoRI and Sal I,which showed that the expression vector PCI- neo-F has been constructed successfully,and thispaved the way to study the Vero cell of mammals. The information about the amplified sequence and the compared knowledge in nucleotide composition, amino acid sequence and systemically de- velopment tree between the amplified F gene sequence and the other ZH-1 F gene,were useful for the further elucidate the genetic background of paramyxovirus type ZH-1.

Key words: Paramyxovirus of brambling chicken, F gene, Sequence analysis, Recombinant plasmid

CLC Number: 

• Q786